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AB127667

Recombinant Human FAPP2 protein

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Recombinant Human FAPP2 protein is a Human Fragment protein, in the 122 to 400 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

FAPP2, PLEKHA8, Pleckstrin homology domain-containing family A member 8, PH domain-containing family A member 8, Phosphatidylinositol-four-phosphate adapter protein 2, Serologically defined breast cancer antigen NY-BR-86, FAPP-2, Phosphoinositol 4-phosphate adapter protein 2, hFAPP2

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96JA3

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 50 µL of water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"30.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":400,"aminoAcidStart":122,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q96JA3","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAPP2 also known as Four-phosphate adaptor protein 2 is an important protein involved in lipid binding and transport. It has a molecular mass of approximately 56 kDa. This protein predominantly localizes in the Golgi apparatus within cells. Its presence is especially prominent in tissues with high levels of secretory activity. FAPP2 functions through its PtdIns(4)P binding capability which facilitates its role in the secretory pathway allowing the transport of glucosylceramide.
Biological function summary

The actions of FAPP2 include the facilitation of lipid transfer and membrane trafficking. It interacts with specific membrane lipids aiding in the formation of transport carriers essential for lipid delivery and glycosphingolipid synthesis. FAPP2 operates within a complex network involving other proteins to ensure accurate lipid sorting and delivery. Its precise role in orchestrating these cellular activities highlights its significance in maintaining membrane composition and signaling.

Pathways

FAPP2 integrates within critical lipid-related processes including the glycosphingolipid biosynthesis and Golgi membrane trafficking pathways. In the glycosphingolipid biosynthesis FAPP2 connects with glucosylceramide and other lipids orchestrating their transport to appropriate cellular destinations. Additionally FAPP2 shares interactions with proteins like CERT and OSBP which are involved in lipid homeostasis and intracellular transport cementing its place as a mediator of critical lipid dynamics.

FAPP2 has associations with disorders related to lipid metabolism such as Gaucher's disease and other lysosomal storage disorders. In these disorders anomalies in lipid transport and metabolism can exacerbate symptoms due to dysfunctional glycosphingolipid synthesis pathways. FAPP2's interactions with proteins like glucosylceramide synthase further illustrate its involvement in disease mechanisms providing a potential target for therapeutic interventions.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation.

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Product protocols

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Target data

Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation.
See full target information PLEKHA8
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Primary Antibodies

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