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AB105613

Recombinant Human FARS2 protein

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Recombinant Human FARS2 protein is a Human Fragment protein, in the 37 to 451 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

FARS1, HSPC320, FARS2, Phenylalanyl-tRNA synthetase, PheRS

1 Images
SDS-PAGE - Recombinant Human FARS2 protein (AB105613)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FARS2 protein (AB105613)

15% SDS PAGE analysis of ab105613 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O95363

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPAAECATQRAPGSVVELLGKSYPQDDHSNLTRKVLTRVGRNLHNQQHHPLWLIKERVKEHFYKQYVGRFGTPLFSVYDNLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAVRLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRHMERTLSQREVRHIHQALQEAAVQLLGVEGRF","proteinLength":"Fragment","predictedMolecularWeight":"50.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":451,"aminoAcidStart":37,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95363","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme known as FARS2 or mitochondrial phenylalanyl-tRNA synthetase 2 plays an important role in mitochondrial protein synthesis. It catalyzes the attachment of phenylalanine to its corresponding tRNA a process essential for translating the genetic code into proteins within mitochondria. FARS2 has a molecular mass of approximately 53 kDa and is mainly expressed in tissues with high energy demands such as brain heart and skeletal muscle. This expression pattern highlights its importance in maintaining proper mitochondrial function.
Biological function summary

FARS2 functions primarily in the mitochondrial translation machinery where it ensures the proper incorporation of phenylalanine into mitochondrial proteins. It operates as a single monomeric enzyme rather than being part of a larger complex highlighting its unique role in mitochondrial biology. Mutations affecting FARS2 can disrupt mitochondrial translation and lead to malfunction of mitochondrial respiratory chain complexes impacting cellular energy production.

Pathways

FARS2 is integral to the mitochondrial translation pathway which plays a critical role in synthesizing proteins required for the oxidative phosphorylation system. This pathway includes various mitochondrial aminoacyl-tRNA synthetases and is closely related to the oxidative phosphorylation pathway. FARS2 interacts functionally with proteins involved in the electron transport chain such as those encoded by the mitochondrial DNA to ensure efficient energy production within cells.

Mutations in FARS2 can lead to neurodegenerative conditions such as combined oxidative phosphorylation deficiency 14 and Alpers syndrome. These disorders highlight the enzyme's role in neural development and function. The disruption in mitochondrial translation caused by faulty FARS2 impacts proteins involved in the oxidative phosphorylation system such as NDUFB8 further exacerbating energy deficiency in affected tissues.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques.

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General info

Function

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.
See full target information FARS2
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