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AB157085

Recombinant human Fas Ligand protein

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(1 Publication)

Recombinant human Fas Ligand protein is a Human protein, in the 103 to 281 aa range, expressed in HEK 293 cells, with >95%, > 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB, FuncS.

View Alternative Names

CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL

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Functional Studies - Recombinant human Fas Ligand protein (AB157085)
  • FuncS

Unknown

Functional Studies - Recombinant human Fas Ligand protein (AB157085)

Activity Assay analysis : Inhibition of ab157085-mediated lysis. Fas (human) : Fc (Human) (rec.) exerts its inhibitory activity in a concentration range of 0.5-5μg/ml in the presence of the enhancer (1μg/ml).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

> 0.1 EU/µg

Expression system

HEK 293 cells

Tags

DDDDK tag N-Terminus

Applications

ELISA, FuncS, WB, SDS-PAGE

applications

Biologically active

Yes

Biological activity

ab157085 binds to Human, Mouse and Rat Fas. ED50: 50ng/ml (A20 cells). Kills Jurkat cells at concentrations of >10ng/ml in the absence of a cross-linker. Cross-linking enhancer increases the activity of rhsFasL approx. 50-fold. Please note: Results using sFasL may differ from those obtained with agonistic antibodies.

Accession

P48023

Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in 100 µL of water

Storage buffer

Constituents: PBS

storage-buffer

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Complementary Products

Primary Antibodies

AB134401

Anti-Fas Ligand antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas Ligand antibody (AB134401)

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Primary Antibodies

AB205606

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB205606)

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Primary Antibodies

AB19857

Anti-Oct4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB19857)

  • 4
  • 45
Proteins & Peptides

AB215626

Recombinant Mouse Fas protein (His tag)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"<p>Use as capture antibody.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(colorimetric, 100ng).</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QIGHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNLPLSHKVYMRNSKYPQDLVMMEGKMMSYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESQTFFGLYKL","proteinLength":null,"predictedMolecularWeight":"32 kDa","actualMolecularWeight":null,"aminoAcidEnd":281,"aminoAcidStart":103,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P48023","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Lyophilized

Additional notes

ab157085 is purified by Multi-Step Chromatography.

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General info

Function

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 26334989, PubMed : 9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 7528780, PubMed : 9228058, PubMed : 9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed : 27806260).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed : 27806260).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.. N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).. Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.. Monoubiquitinated.

Subcellular localisation

Lysosome lumen

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Product protocols

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Target data

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 26334989, PubMed : 9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 7528780, PubMed : 9228058, PubMed : 9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed : 27806260).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed : 27806260).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
See full target information FASLG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0273843 PubMed36054185

2022

Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone.

Applications

Unspecified application

Species

Unspecified reactive species

Ruomei Li,Sabin Bhandari,Inigo Martinez-Zubiaurre,Jack-Ansgar Bruun,Ilona Urbarova,Bård Smedsrød,Jaione Simón-Santamaría,Karen Kristine Sørensen
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