Recombinant human Fas Ligand protein is a Human protein, in the 103 to 281 aa range, expressed in HEK 293, with >95% purity, > 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB, FuncS.
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- PloS one 17:e02738432022Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuomei Li,Sabin Bhandari,Inigo Martinez-Zubiaurre,Jack-Ansgar Bruun,Ilona Urbarova,Bård Smedsrød,Jaione Simón-Santamaría,Karen Kristine SørensenPubMed 36054185
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- > 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
Q I G H P S P P P E K K E L R K V A H L T G K S N S R S M P L E W E D T Y G I V L L S G V K Y K K G G L V I N E T G L Y F V Y S K V Y F R G Q S C N N L P L S H K V Y M R N S K Y P Q D L V M M E G K M M S Y C T T G Q M W A R S S Y L G A V F N L T S A D H L Y V N V S E L S L V N F E E S Q T F F G L Y K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1 µg/mL | Notes Use as capture antibody. |
Application WB | Reactivity Reacts | Dilution info - | Notes (colorimetric, 100ng). |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:7528780, PubMed:9228058, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260). Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed:27806260). FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
Alternative names
CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL
Recommended products
Recombinant human Fas Ligand protein is a Human protein, in the 103 to 281 aa range, expressed in HEK 293, with >95% purity, > 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- > 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB, FuncS
- Biologically active
- Yes
- Biological activity
- ab157085 binds to Human, Mouse and Rat Fas. ED50: 50ng/ml (A20 cells). Kills Jurkat cells at concentrations of >10ng/ml in the absence of a cross-linker. Cross-linking enhancer increases the activity of rhsFasL approx. 50-fold. Please note: Results using sFasL may differ from those obtained with agonistic antibodies.
- Accession
- P48023-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in 100 µL of water
- Concentration
- Storage buffer
Constituents: 99% PBS
Sequence info
Amino acid sequence
- Q I G H P S P P P E K K E L R K V A H L T G K S N S R S M P L E W E D T Y G I V L L S G V K Y K K G G L V I N E T G L Y F V Y S K V Y F R G Q S C N N L P L S H K V Y M R N S K Y P Q D L V M M E G K M M S Y C T T G Q M W A R S S Y L G A V F N L T S A D H L Y V N V S E L S L V N F E E S Q T F F G L Y K L
- Accession
- P48023
- Predicted molecular weight
- 32 kDa
- Amino acids
- 103 to 281
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
ab157085 is purified by Multi-Step Chromatography.
General info
- Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:7528780, PubMed:9228058, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
- Subcellular localisation
- Lysosome lumen, Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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Full details and terms and conditions can be found here:
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Functional Studies - Recombinant human Fas Ligand protein (ab157085)
Activity Assay analysis: Inhibition of ab157085-mediated lysis. Fas (human):Fc (Human) (rec.) exerts its inhibitory activity in a concentration range of 0.5-5μg/ml in the presence of the enhancer (1μg/ml).
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