Recombinant human Fas protein is a Human Fragment protein, in the 26 to 173 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
Q V T D I N S K G L E L R K T V T T V E T Q N L E G L H H D G Q F C H K P C P P G E R K A R D C T V N G D E P D C V P C Q E G K E Y T D K A H F S S K C R R C R L C D E G H G L E V E I N C T R T Q N T K C R C K P N F F C N S T V C E H C D P C T K C E H G I I K E C T L T S N T K C K E E G S R S N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes ab155635 migrates as 45-55 kDa in reduced SDS-PAGE resulting from glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Alternative names
CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor
Recommended products
Recombinant human Fas protein is a Human Fragment protein, in the 26 to 173 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells. The ED50 for this effect is typically 8-35 pg/ml in the presence of 2 ng/ml recombinant human Fas Ligand.
- Accession
- P25445-1
- Animal free
- No
- Species
- Human
- Reconstitution
- It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- Q V T D I N S K G L E L R K T V T T V E T Q N L E G L H H D G Q F C H K P C P P G E R K A R D C T V N G D E P D C V P C Q E G K E Y T D K A H F S S K C R R C R L C D E G H G L E V E I N C T R T Q N T K C R C K P N F F C N S T V C E H C D P C T K C E H G I I K E C T L T S N T K C K E E G S R S N
- Accession
- P25445
- Protein length
- Fragment
- Predicted molecular weight
- 42.8 kDa
- Amino acids
- 26 to 173
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
- Post-translational modifications
(Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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3 product images
SDS-PAGE - Recombinant human Fas protein (ab155635)
Human Fas, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Functional Studies - Recombinant human Fas protein (ab155635)
Immobilized Human Fas Ligand, His Tag at 2 µg/mL (100 µL/well)can bind Human Fas, Fc Tag with a linear range of 4-31 ng/mL.
SDS-PAGE - Recombinant human Fas protein (ab155635)
SDS PAGE analysis of reduced ab155635 stained overnight with Coomassie Blue.
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