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AB99240

Recombinant Human Fascin protein (His tag N-Terminus)

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Recombinant Human Fascin protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 493 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

FAN1, HSN, SNL, FSCN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55

1 Images
SDS-PAGE - Recombinant Human Fascin protein (AB99240)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Fascin protein (AB99240)

15% SDS-PAGE showing ab99240 at approximately 56.7kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q16658

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY","proteinLength":"Full Length","predictedMolecularWeight":"56.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":493,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16658","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab99240 is purified using conventional chromatography techniques.

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General info

Function

Actin-binding protein that contains 2 major actin binding sites (PubMed : 21685497, PubMed : 23184945). Organizes filamentous actin into parallel bundles (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed : 22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed : 22155786).

Sequence similarities

Belongs to the fascin family.

Post-translational modifications

Phosphorylation at Ser-39 inhibits actin-binding (PubMed:8647875, PubMed:8999969). Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF (PubMed:22155786).

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Actin-binding protein that contains 2 major actin binding sites (PubMed : 21685497, PubMed : 23184945). Organizes filamentous actin into parallel bundles (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed : 22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed : 22155786).
See full target information FSCN1
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB126772

Anti-Fascin antibody [EP5902]

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Immunocytochemistry/ Immunofluorescence - Anti-Fascin antibody [EP5902] (AB126772)

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Secondary Antibodies

AB150121

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Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

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