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AB286057

Recombinant human FBP1 protein (Active)

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Recombinant human FBP1 protein (Active) is a Human Full Length protein, in the 1 to 388 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

FBP, FBP1, FBPase 1, Liver FBPase

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Functional Studies - Recombinant human FBP1 protein (Active) (AB286057)
  • FuncS

Supplier Data

Functional Studies - Recombinant human FBP1 protein (Active) (AB286057)

Enzyme activity analysis of ab286057. Enzyme's activity was assayed in 50 mM Tris pH 8.0, 5 mM MgCl2 at 37°C for 30 minute with fluorometric reporter probe at Ex/Em 535/587 nm. The specific activity of the enzyme is 1 U/mg.

SDS-PAGE - Recombinant human FBP1 protein (Active) (AB286057)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human FBP1 protein (Active) (AB286057)

SDS-PAGE analysis of ab286057 loaded under reducing conditions and stained with Coomassie Blue.

Lane M : Molecular weight marker

Lane 2 : ab286057 at 2.5 μg

Lane 3 : ab286057 at 5 μg

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is ≥ 1 U/mg.

One unit is the amount of enzyme that will hydrolyze 1.0 μmole of fructose 1,6-bisphosphate to fructose 6-phosphate at pH 8.0 and 37°C for 30 minutes.

Accession

P09467

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in deionized water to a final concentration of 1.5 µg/ml. The reconstituted enzyme can be stored at 4°C for up to 2 weeks. For long term storage, aliquot and store at -70°C.

Storage buffer

Constituents: PBS, 8% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called P1269 Fructose 1,6-bisphophatase 1, Active, Human Recombinant. P1269-50 is the same size as the 50 μg size of ab286057.
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Sequence info

[{"sequence":"MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ","proteinLength":"Full Length","predictedMolecularWeight":"36.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":388,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09467","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBP1 also known as fructose-16-bisphosphatase 1 is an enzyme with a molecular mass of approximately 37 kDa. It plays a mechanical role in catalyzing the conversion of fructose-16-bisphosphate to fructose 6-phosphate in the gluconeogenesis pathway. This enzyme expresses primarily in the liver but also in kidneys and muscles reflecting its key role in glucose metabolism.
Biological function summary

The function of FBP1 involves maintaining glucose homeostasis which is essential for energy balance. FBP1 is part of the gluconeogenesis pathway a process that synthesizes glucose from non-carbohydrate sources. This enzyme acts independently and does not usually form part of larger protein complexes but its activity significantly impacts liver metabolism and energy production in low-glucose conditions.

Pathways

FBP1 finds its primary involvement in the gluconeogenesis pathway. This roles complements those of other enzymes such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase which also contribute to glucose synthesis. FBP1's activity supports glucose production providing glucose for bloodstream circulation when dietary intake is low.

FBP1's function directly relates to conditions such as fructose 16-bisphosphatase deficiency and has implications in type 2 diabetes. Deficient FBP1 can lead to life-threatening hypoglycemia demonstrating the need for energy regulation by this enzyme. Its regulation intersects with insulin signaling pathways with proteins like insulin receptor substrate (IRS) playing roles in modulating its activity in metabolic disorders.
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Specifications

Form

Lyophilized

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General info

Function

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

Sequence similarities

Belongs to the FBPase class 1 family.

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Product protocols

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Target data

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
See full target information FBP1
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