Recombinant human FBP2 protein is a Human Full Length protein, in the 1 to 339 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M T D R S P F E T D M L T L T R Y V M E K G R Q A K G T G E L T Q L L N S M L T A I K A I S S A V R K A G L A H L Y G I A G S V N V T G D E V K K L D V L S N S L V I N M V Q S S Y S T C V L V S E E N K D A I I T A K E K R G K Y V V C F D P L D G S S N I D C L A S I G T I F A I Y R K T S E D E P S E K D A L Q C G R N I V A A G Y A L Y G S A T L V A L S T G Q G V D L F M L D P A L G E F V L V E K D V K I K K K G K I Y S L N E G Y A K Y F D A A T T E Y V Q K K K F P E D G S A P Y G A R Y V G S M V A D V H R T L V Y G G I F L Y P A N Q K S P K G K L R L L Y E C N P V A Y I I E Q A G G L A T T G T Q P V L D V K P E A I H Q R V P L I L G S P E D V Q E Y L T C V Q K N Q A G S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.
Alternative names
FBPase 2, Muscle FBPase, FBP2
Recommended products
Recombinant human FBP2 protein is a Human Full Length protein, in the 1 to 339 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 1,500 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.
- Accession
- O00757-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M T D R S P F E T D M L T L T R Y V M E K G R Q A K G T G E L T Q L L N S M L T A I K A I S S A V R K A G L A H L Y G I A G S V N V T G D E V K K L D V L S N S L V I N M V Q S S Y S T C V L V S E E N K D A I I T A K E K R G K Y V V C F D P L D G S S N I D C L A S I G T I F A I Y R K T S E D E P S E K D A L Q C G R N I V A A G Y A L Y G S A T L V A L S T G Q G V D L F M L D P A L G E F V L V E K D V K I K K K G K I Y S L N E G Y A K Y F D A A T T E Y V Q K K K F P E D G S A P Y G A R Y V G S M V A D V H R T L V Y G G I F L Y P A N Q K S P K G K L R L L Y E C N P V A Y I I E Q A G G L A T T G T Q P V L D V K P E A I H Q R V P L I L G S P E D V Q E Y L T C V Q K N Q A G S
- Accession
- O00757
- Protein length
- Full Length
- Predicted molecular weight
- 39 kDa
- Amino acids
- 1 to 339
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab208316 was purified using conventional chromatography techniques.
General info
- Function
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.
- Sequence similarities
Belongs to the FBPase class 1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FBP2 also known as fructose-16-bisphosphatase 2 is an enzyme that catalyzes the conversion of fructose-16-bisphosphate to fructose 6-phosphate. This reaction is important for the process of gluconeogenesis. FBP2 weighs around 37.6 kDa and is expressed in various tissues including the liver and kidney where it supports energy metabolism by synthesizing glucose from non-carbohydrate precursors.
- Biological function summary
The enzyme plays a role in glucose homeostasis by regulating gluconeogenesis. It is a part of the fructose-16-bisphosphatase complex that controls the balance between glycolysis and gluconeogenesis. FBP2 activity helps to maintain blood glucose levels particularly during periods of fasting or intense physical activity. By adjusting the rate of glucose production and consumption the enzyme contributes to metabolic flexibility.
- Pathways
FBP2 functions in the regulation of the gluconeogenesis pathway. This pathway is key in maintaining normal blood glucose levels and the enzyme acts in coordination with related enzymes like glucose-6-phosphatase. FBP2 is involved in the balance between glycolysis and gluconeogenesis partnering with enzymes such as phosphofructokinase which perform opposite functions to regulate energy production and consumption. This fine-tuning of energy pathways ensures that cells have a stable supply of glucose adjusting for factors such as nutrient availability and hormonal signals.
- Associated diseases and disorders
FBP2 has connections to metabolic disorders like type 2 diabetes and glycogen storage diseases. Impaired FBP2 activity can disrupt glucose homeostasis leading to dysregulated blood sugar levels characteristic of type 2 diabetes. Additionally the activity of FBP2 can influence the function of other proteins like insulin receptor substrates impacting insulin signaling pathways. Dysfunctions in these pathways can exacerbate insulin resistance and metabolic syndromes emphasizing the significance of FBP2 in maintaining metabolic health.
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1 product image
SDS-PAGE - Recombinant human FBP2 protein (ab208316)
15% SDS-PAGE analysis of ab208316 (3μg).
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