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AB161801

Recombinant Human FBXL2 protein (GST tag N-Terminus)

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Recombinant Human FBXL2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 423 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

FBL2, FBL3, FBXL2, F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2, F-box protein FBL2/FBL3

1 Images
SDS-PAGE - Recombinant Human FBXL2 protein (GST tag N-Terminus) (AB161801)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FBXL2 protein (GST tag N-Terminus) (AB161801)

ab161801 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q9UKC9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Recombinant Human Zinc finger protein 691 (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Zinc finger protein 691 (GST tag N-Terminus) (AB162402)

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Primary Antibodies

AB153842

Anti-FBXL2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL2 antibody - N-terminal (AB153842)

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Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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Proteins & Peptides

AB161165

Recombinant Human WASF3 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human WASF3 protein (GST tag N-Terminus) (AB161165)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MVFSNNDEGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAWNILALDGSNWQRIDLFNFQTDVEGRVVENISKRCGGFLRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCYSLSRFCSKLKHLDLTSCVSITNSSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHLTDAGFTLLARNCHELEKMDLEECILITDSTLIQLSIHCPKLQALSLSHCELITDDGILHLSNSTCGHERLRVLELDNCLLITDVALEHLENCRGLERLELYDCQQVTRAGIKRMRAQLPHVKVHAYFAPVTPPTAVAGSGQRLCRCCVIL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":423,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9UKC9","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXL2 also known as F-box and leucine-rich repeat protein 2 is involved in protein degradation mechanisms. It is part of a SCF (SKP1-Cullin-F-box) complex and functions as a ubiquitin ligase. FBXL2 targets proteins for ubiquitination tagging them for proteasomal degradation. Weighing approximately 49 kDa FBXL2 shows expression in various tissues including the liver heart and brain. Its role in maintaining protein homeostasis highlights its importance in cellular processes.
Biological function summary

The FBXL2 protein maintains cellular protein quality by regulating the turnover of specific substrates. It forms a complex with SKP1 and Cullin-2 in the ubiquitin-proteasome pathway. FBXL2 mediates the degradation of important regulatory proteins influencing the cell cycle and signal transduction. This activity impacts cellular responses to stress and environmental changes ensuring proper cellular adaptation.

Pathways

FBXL2 participates in the cell cycle and apoptosis regulation pathways. It intersects with the PI3K/Akt signaling pathway where it interacts with proteins like Cyclin D1 and Cdk inhibitors playing a part in cell proliferation and survival. These interactions demonstrate FBXL2's important role in controlling cell growth and division affecting how cells respond to intrinsic and extrinsic signals.

FBXL2 connects to cancer and neurodegeneration. In cancer FBXL2 may impact tumor progression due to its role in modulating oncogenic pathways interacting with proteins like mTOR which regulate cell growth. Additionally in neurodegenerative diseases FBXL2 affects protein aggregation processes which are relevant in conditions like Alzheimer's disease by targeting specific substrates for degradation. This connection to key disease mechanisms highlights FBXL2 as a significant focus for therapeutic research.
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Specifications

Form

Liquid

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General info

Function

Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 22020328, PubMed : 22323446, PubMed : 31209342). Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin (PubMed : 22020328, PubMed : 22323446). This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin (PubMed : 22020328, PubMed : 22323446). Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0) (PubMed : 22020328, PubMed : 22323446). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (PubMed : 23604317). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity). The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6) (By similarity). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3 (PubMed : 26037928). The GGTase-3 complex, composed of PTAR1 and RABGGTB, geranylgeranylates and targets FBXL2 to the cellular membranes, where FBXL2 forms part of the SCF(FBXL2) complex that mediates the degradation of membrane-anchored proteins (PubMed : 31209342, PubMed : 32128853).

Post-translational modifications

Phosphorylated by GSK-beta (GSK3B), promoting recognition by FBXO3, leading to its ubiquitination by the SCF(FBXO3) complex.. Ubiquitinated at Lys-201 by the SCF(FBXO3) complex in response to lipopolysaccharide (LPS), leading to its degradation by the proteasome.. Geranylgeranylation at Cys-420 is mediated by the GGTase-3 complex and is required for its association with cell membranes and the recruitment of substrates to the active SCF(FBXL2) complex.

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Product protocols

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Target data

Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 22020328, PubMed : 22323446, PubMed : 31209342). Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin (PubMed : 22020328, PubMed : 22323446). This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin (PubMed : 22020328, PubMed : 22323446). Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0) (PubMed : 22020328, PubMed : 22323446). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (PubMed : 23604317). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity). The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6) (By similarity). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3 (PubMed : 26037928). The GGTase-3 complex, composed of PTAR1 and RABGGTB, geranylgeranylates and targets FBXL2 to the cellular membranes, where FBXL2 forms part of the SCF(FBXL2) complex that mediates the degradation of membrane-anchored proteins (PubMed : 31209342, PubMed : 32128853).
See full target information FBXL2
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