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AB180282

Recombinant Human FBXO2 protein (His tag N-Terminus)

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Recombinant Human FBXO2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 296 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

FBX2, FBXO2, F-box only protein 2

1 Images
SDS-PAGE - Recombinant Human FBXO2 protein (His tag N-Terminus) (AB180282)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human FBXO2 protein (His tag N-Terminus) (AB180282)

15% SDS-PAGE analysis of ab180282 (3μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9UK22

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMDGDGDPESVGQPEEASPEEQPEEASAEEERPEDQQEEEAAAAAAYLDELPEPLLLRVLAALPAAELVQACRLVCLRWKELVDGAPLWLLKCQQEGLVPEGGVEEERDHWQQFYFLSKRRRNLLRNPCGEEDLEGWCDVEHGGDGWRVEELPGDSGVEFTHDESVKKYFASSFEWCRKAQVIDLQAEGYWEELLDTTQPAIVVKDWYSGRSDAGCLYELTVKLLSEHENVLAEFSSGQVAVPQDSDGGGWMEISHTFTDYGPGVRFVRFEHGGQDSVYWKGWFGARVTNSSVWVEP","proteinLength":"Full Length","predictedMolecularWeight":"35.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":296,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UK22","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBXO2 also known as F-box protein 2 is part of the F-box protein family. This protein plays a mechanical role in recognizing specific substrates for ubiquitination a process important for protein degradation. FBXO2 weighs around 32 kDa and mainly expresses in the brain where it regulates protein levels. This localization suggests its significance in neural protein management.
Biological function summary

FBXO2 participates in forming the SCF (SKP1-CUL1-F-box protein) complex a multi-protein E3 ubiquitin ligase complex. It targets glycosylated proteins for degradation which helps control glycoprotein homeostasis in cells. FBXO2 binds to N-linked glycoproteins indicating its specificity towards glycan recognition and processing. This function makes it important for maintaining cellular protein balance.

Pathways

FBXO2 is involved in the ubiquitin-proteasome pathway which is essential for protein turnover and quality control. It also plays a role in the endoplasmic reticulum-associated degradation (ERAD) pathway which manages misfolded proteins. FBXO2 collaborates with proteins such as β-transducin repeat-containing protein (β-TrCP) within these pathways showing its interaction in various cellular mechanisms.

FBXO2 has connections to neurodegenerative disorders and cancer. Aberrations in FBXO2 function are linked to Alzheimer's disease due to its role in protein degradation in neurons. In cancer FBXO2 alteration affects glycoprotein regulation potentially leading to tumor progression and metastasis. The protein relates to other F-box proteins which also influence cellular stability and disease progression.
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Specifications

Form

Liquid

Additional notes

ab180282 is purified using conventional chromatography techniques

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General info

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type (By similarity).

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Product protocols

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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type (By similarity).
See full target information FBXO2
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