Recombinant Human FBXO9 protein is a Human Full Length protein, in the 1 to 437 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A E A E E D C H S D T V R A D D D E E N E S P A E T D L Q A Q L Q M F R A Q W M F E L A P G V S S S N L E N R P C R A A R G S L Q K T S A D T K G K Q E Q A K E E K A R E L F L K A V E E E Q N G A L Y E A I K F Y R R A M Q L V P D I E F K I T Y T R S P D G D G V G N S Y I E D N D D D S K M A D L L S Y F Q Q Q L T F Q E S V L K L C Q P E L E S S Q I H I S V L P M E V L M Y I F R W V V S S D L D L R S L E Q L S L V C R G F Y I C A R D P E I W R L A C L K V W G R S C I K L V P Y T S W R E M F L E R P R V R F D G V Y I S K T T Y I R Q G E Q S L D G F Y R A W H Q V E Y Y R Y I R F F P D G H V M M L T T P E E P Q S I V P R L R T R N T R T D A I L L G H Y R L S Q D T D N Q T K V F A V I T K K K E E K P L D Y K Y R Y F R R V P V Q E A D Q S F H V G L Q L C S S G H Q R F N K L I W I H H S C H I T Y K S T G E T A V S A F E I D K M Y T P L F F A R V R S Y T A F S E R P L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability (PubMed:23263282, PubMed:34480022). Ubiquitinates mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is the activated due to the relief of feedback inhibition by mTORC1 (PubMed:23263282). Plays a role in maintaining epithelial cell survival by regulating the turn-over of chromatin modulator PRMT4 through ubiquitination and degradation by the proteasomal pathway (PubMed:34480022). Regulates also PPARgamma stability by facilitating PPARgamma/PPARG ubiquitination and thereby plays a role in adipocyte differentiation (By similarity).
Alternative names
FBX9, VCIA1, FBXO9, F-box only protein 9, Cross-immune reaction antigen 1, Renal carcinoma antigen NY-REN-57
Recommended products
Recombinant Human FBXO9 protein is a Human Full Length protein, in the 1 to 437 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9UK97-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A E A E E D C H S D T V R A D D D E E N E S P A E T D L Q A Q L Q M F R A Q W M F E L A P G V S S S N L E N R P C R A A R G S L Q K T S A D T K G K Q E Q A K E E K A R E L F L K A V E E E Q N G A L Y E A I K F Y R R A M Q L V P D I E F K I T Y T R S P D G D G V G N S Y I E D N D D D S K M A D L L S Y F Q Q Q L T F Q E S V L K L C Q P E L E S S Q I H I S V L P M E V L M Y I F R W V V S S D L D L R S L E Q L S L V C R G F Y I C A R D P E I W R L A C L K V W G R S C I K L V P Y T S W R E M F L E R P R V R F D G V Y I S K T T Y I R Q G E Q S L D G F Y R A W H Q V E Y Y R Y I R F F P D G H V M M L T T P E E P Q S I V P R L R T R N T R T D A I L L G H Y R L S Q D T D N Q T K V F A V I T K K K E E K P L D Y K Y R Y F R R V P V Q E A D Q S F H V G L Q L C S S G H Q R F N K L I W I H H S C H I T Y K S T G E T A V S A F E I D K M Y T P L F F A R V R S Y T A F S E R P L
- Accession
- Q9UK97
- Protein length
- Full Length
- Amino acids
- 1 to 437
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability (PubMed:23263282, PubMed:34480022). Ubiquitinates mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is the activated due to the relief of feedback inhibition by mTORC1 (PubMed:23263282). Plays a role in maintaining epithelial cell survival by regulating the turn-over of chromatin modulator PRMT4 through ubiquitination and degradation by the proteasomal pathway (PubMed:34480022). Regulates also PPARgamma stability by facilitating PPARgamma/PPARG ubiquitination and thereby plays a role in adipocyte differentiation (By similarity).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FBXO9 also known as F-box only protein 9 functions as part of the SCF (SKP1-cullin-F-box) ubiquitin ligase complex. This complex tags proteins for degradation by attaching ubiquitin molecules. FBXO9 specifically acts as a substrate recognition component targeting proteins for ubiquitination and subsequent proteasomal degradation. The mass of FBXO9 is around 47 kDa. It is expressed in various tissues with a significant presence in the lung and spleen.
- Biological function summary
FBXO9 participates in the regulation of protein stability and turnover. As a component of the SCF complex it plays a role in controlling cell cycle progression and signal transduction. Its function in recognizing and binding specific proteins identifies them for ubiquitin-mediated degradation regulating protein levels within cells. This process is important for maintaining cellular homeostasis and responding to changes in the cellular environment.
- Pathways
FBXO9 interacts with processes involved in protein degradation and cell cycle regulation. It is a part of the ubiquitin-proteasome pathway important for removing damaged or misfolded proteins. In addition FBXO9 influences the cell cycle pathway by targeting regulatory proteins for degradation affecting their availability and function. It works alongside proteins such as SKP1 and cullin-1 in these pathways ensuring precise control over cell cycle progression.
- Associated diseases and disorders
FBXO9 has links to certain types of cancers and neurodegenerative diseases. Aberrations in its function may lead to disruptions in protein homeostasis contributing to tumorigenesis through unchecked proliferation due to altered cell cycle regulation. In neurodegenerative diseases FBXO9's role in protein degradation becomes significant as accumulation of malfunctioning proteins can lead to cell death. Interactions with proteins like p27 a cyclin-dependent kinase inhibitor highlight its importance in these disease contexts.
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1 product image
SDS-PAGE - Recombinant Human FBXO9 protein (ab161954)
ab161954 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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