Recombinant Human Fbxw7 protein is a Human Full Length protein, in the 1 to 707 aa range, expressed in Cell free, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M N Q E L L S V G S K R R R T G G S L R G N P S S S Q V D E E Q M N R V V E E E Q Q Q Q L R Q Q E E E H T A R N G E V V G V E P R P G G Q N D S Q Q G Q L E E N N N R F I S V D E D S S G N Q E E Q E E D E E H A G E Q D E E D E E E E E M D Q E S D D F D Q S D D S S R E D E H T H T N S V T N S S S I V D L P V H Q L S S P F Y T K T T K M K R K L D H G S E V R S F S L G K K P C K V S E Y T S T T G L V P C S A T P T T F G D L R A A N G Q G Q Q R R R I T S V Q P P T G L Q E W L K M F Q S W S G P E K L L A L D E L I D S C E P T Q V K H M M Q V I E P Q F Q R D F I S L L P K E L A L Y V L S F L E P K D L L Q A A Q T C R Y W R I L A E D N L L W R E K C K E E G I D E P L H I K R R K V I K P G F I H S P W K S A Y I R Q H R I D T N W R R G E L K S P K V L K G H D D H V I T C L Q F C G N R I V S G S D D N T L K V W S A V T G K C L R T L V G H T G G V W S S Q M R D N I I I S G S T D R T L K V W N A E T G E C I H T L Y G H T S T V R C M H L H E K R V V S G S R D A T L R V W D I E T G Q C L H V L M G H V A A V R C V Q Y D G R R V V S G A Y D F M V K V W D P E T E T C L H T L Q G H T N R V Y S L Q F D G I H V V S G S L D T S I R V W D V E T G N C I H T L T G H Q S L T S G M E L K D N I L V S G N A D S T V K I W D I K T G Q C L Q T L Q G P N K H Q S A V T C L Q F N K N F V I T S S D D G T V K L W D L K T G E F I R N L V T L E S G G S G G V V W R I R A S N T K L V C A V G S R N G T E E T K L L V L D F D V D M K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:17434132, PubMed:22748924, PubMed:26976582, PubMed:28727686, PubMed:34741373, PubMed:35395208). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination (PubMed:17434132, PubMed:22748924, PubMed:26774286, PubMed:26976582, PubMed:28727686, PubMed:34741373). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1 (PubMed:11565034, PubMed:11585921, PubMed:12354302, PubMed:14739463, PubMed:15103331, PubMed:17558397, PubMed:17873522, PubMed:22608923, PubMed:22748924, PubMed:25775507, PubMed:25897075, PubMed:26976582, PubMed:28007894, PubMed:28727686, PubMed:29149593, PubMed:34102342). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed:27238018). Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage (PubMed:26774286). The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining (PubMed:26774286).
Alternative names
FBW7, FBX30, SEL10, FBXW7, F-box/WD repeat-containing protein 7, Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4, hAgo
Recommended products
Recombinant Human Fbxw7 protein is a Human Full Length protein, in the 1 to 707 aa range, expressed in Cell free, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q969H0-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.79% Sodium chloride, 0.14% Disodium hydrogenorthophosphate, 0.024% Potassium phosphate monobasic, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M N Q E L L S V G S K R R R T G G S L R G N P S S S Q V D E E Q M N R V V E E E Q Q Q Q L R Q Q E E E H T A R N G E V V G V E P R P G G Q N D S Q Q G Q L E E N N N R F I S V D E D S S G N Q E E Q E E D E E H A G E Q D E E D E E E E E M D Q E S D D F D Q S D D S S R E D E H T H T N S V T N S S S I V D L P V H Q L S S P F Y T K T T K M K R K L D H G S E V R S F S L G K K P C K V S E Y T S T T G L V P C S A T P T T F G D L R A A N G Q G Q Q R R R I T S V Q P P T G L Q E W L K M F Q S W S G P E K L L A L D E L I D S C E P T Q V K H M M Q V I E P Q F Q R D F I S L L P K E L A L Y V L S F L E P K D L L Q A A Q T C R Y W R I L A E D N L L W R E K C K E E G I D E P L H I K R R K V I K P G F I H S P W K S A Y I R Q H R I D T N W R R G E L K S P K V L K G H D D H V I T C L Q F C G N R I V S G S D D N T L K V W S A V T G K C L R T L V G H T G G V W S S Q M R D N I I I S G S T D R T L K V W N A E T G E C I H T L Y G H T S T V R C M H L H E K R V V S G S R D A T L R V W D I E T G Q C L H V L M G H V A A V R C V Q Y D G R R V V S G A Y D F M V K V W D P E T E T C L H T L Q G H T N R V Y S L Q F D G I H V V S G S L D T S I R V W D V E T G N C I H T L T G H Q S L T S G M E L K D N I L V S G N A D S T V K I W D I K T G Q C L Q T L Q G P N K H Q S A V T C L Q F N K N F V I T S S D D G T V K L W D L K T G E F I R N L V T L E S G G S G G V V W R I R A S N T K L V C A V G S R N G T E E T K L L V L D F D V D M K
- Accession
- Q969H0
- Protein length
- Full Length
- Predicted molecular weight
- 83.7 kDa
- Amino acids
- 1 to 707
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:17434132, PubMed:22748924, PubMed:26976582, PubMed:28727686, PubMed:34741373, PubMed:35395208). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination (PubMed:17434132, PubMed:22748924, PubMed:26774286, PubMed:26976582, PubMed:28727686, PubMed:34741373). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1 (PubMed:11565034, PubMed:11585921, PubMed:12354302, PubMed:14739463, PubMed:15103331, PubMed:17558397, PubMed:17873522, PubMed:22608923, PubMed:22748924, PubMed:25775507, PubMed:25897075, PubMed:26976582, PubMed:28007894, PubMed:28727686, PubMed:29149593, PubMed:34102342). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed:27238018). Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage (PubMed:26774286). The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining (PubMed:26774286).
- Post-translational modifications
Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923). Phosphorylated by ATM at Ser-26 in response to DNA damage, promoting recruitment to DNA damage sites and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4 (PubMed:26774286).
- Subcellular localisation
- Nucleus, Nucleoplasm, Nucleolus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Fbxw7 protein (ab289752)
SDS-PAGE analysis of ab289752
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com