Recombinant Human FDFT1 protein (denatured) is a Human Fragment protein, in the 1 to 283 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E F V K C L G H P E E F Y N L V R F R I G G K R K V M P K M D Q D S L S S S L K T C Y K Y L N Q T S R S F A A V I Q A L D G E M R N A V C I F Y L V L R A L D T L E D D M T I S V E K K V P L L H N F H S F L Y Q P D W R F M E S K E K D R Q V L E D F P T I S L E F R N L A E K Y Q T V I A D I C R R M G I G M A E F L D K H V T S E Q E W D K Y C H Y V A G L V G I G L S R L F S A S E F E D P L V G E D T E R A N S M G L F L Q K T N I I R D Y L E D Q Q G G R E F W P Q E V W S R Y V K K L G D F A K P E N I D L A V Q C L N E L I T N A L H H I P D V I T Y L S R L R N Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
Alternative names
Squalene synthase, SQS, SS, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase, Farnesyl-diphosphate farnesyltransferase 1, FDFT1
Recommended products
Recombinant Human FDFT1 protein (denatured) is a Human Fragment protein, in the 1 to 283 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P37268-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M E F V K C L G H P E E F Y N L V R F R I G G K R K V M P K M D Q D S L S S S L K T C Y K Y L N Q T S R S F A A V I Q A L D G E M R N A V C I F Y L V L R A L D T L E D D M T I S V E K K V P L L H N F H S F L Y Q P D W R F M E S K E K D R Q V L E D F P T I S L E F R N L A E K Y Q T V I A D I C R R M G I G M A E F L D K H V T S E Q E W D K Y C H Y V A G L V G I G L S R L F S A S E F E D P L V G E D T E R A N S M G L F L Q K T N I I R D Y L E D Q Q G G R E F W P Q E V W S R Y V K K L G D F A K P E N I D L A V Q C L N E L I T N A L H H I P D V I T Y L S R L R N Q
- Accession
- P37268
- Protein length
- Fragment
- Predicted molecular weight
- 35.4 kDa
- Amino acids
- 1 to 283
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
- Sequence similarities
Belongs to the phytoene/squalene synthase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FDFT1 also known as squalene synthase catalyzes the first committed step in cholesterol biosynthesis. This enzyme functions as a dimer and has a molecular weight of approximately 47 kDa. FDFT1 is widely expressed in the liver where cholesterol synthesis primarily occurs but you can also find it present in many tissues throughout the body. As a membrane-bound protein it anchors to the endoplasmic reticulum where it carries out its enzymatic role.
- Biological function summary
FDFT1 facilitates the conversion of farnesyl diphosphate to squalene a critical precursor in sterol and cholesterol synthesis. This enzyme does not work in isolation but acts as a part of the larger sterol biosynthesis pathway. The activity of FDFT1 aligns with key metabolic demands particularly in tissues with high rates of cell division or hormone production making it essential for maintaining cellular sterol homeostasis.
- Pathways
FDFT1 is an important enzyme in the mevalonate pathway and cholesterol biosynthesis pathway. This enzyme interacts closely with proteins such as HMG-CoA reductase and lanosterol synthase which are pivotal in these pathways. This relationship ensures the proper flow from acetyl-CoA to cholesterol and other isoprenoids critical for cell membrane integrity and signaling functions.
- Associated diseases and disorders
Disruptions in FDFT1 activity can lead to conditions like hypercholesterolemia and related cardiovascular diseases. Excess or deficiency in its function impacts cholesterol levels directly making it a target for cholesterol-lowering therapies. FDFT1's activity also connects to the proteins NPC1 and LDL receptor which play a role in cholesterol transport and uptake further linking it to pathways that contribute to atherosclerosis when dysregulated.
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1 product image
SDS-PAGE - Recombinant Human FDFT1 protein (denatured) (ab183258)
15% SDS-PAGE analysis of ab183258 (3μg).
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