Recombinant Human Ferritin Heavy Chain protein is a Human Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA, WB.
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- Cell death discovery 8:4262022Ferrostatin-1 alleviates tissue and cell damage in diabetic retinopathy by improving the antioxidant capacity of the Xc-GPX4 system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingzhi Shao,Zhouxian Bai,Lirong Zhang,Fengyan ZhangPubMed 36284090
- Annals of the rheumatic diseases 71:943-72012Association of ferritin autoantibodies with giant cell arteritis/polymyalgia rheumatica.Applications:ELISAReactive species:Unspecified reactive speciesN T Baerlecken et. al.PubMed 22228484
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M T T A S T S Q V R Q N Y H Q D S E A A I N R Q I N L E L Y A S Y V Y L S M S Y Y F D R D D V A L K N F A K Y F L H Q S H E E R E H A E K L M K L Q N Q R G G R I F L Q D I K K P D C D D W E S G L N A M E C A L H L E K N V N Q S L L E L H K L A T D K N D P H L C D F I E T H Y L N E Q V K A I K E L G D H V T N L R K M G A P E S G L A E Y L F D K H T L G D S D N E S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab78877 can be used as a WB positive control in conjunction with Anti-Ferritin Heavy Chain antibody ab65080. |
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293).
Alternative names
FTH, FTHL6, OK/SW-cl.84, PIG15, FTH1, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein
Recommended products
Recombinant Human Ferritin Heavy Chain protein is a Human Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P02794-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M T T A S T S Q V R Q N Y H Q D S E A A I N R Q I N L E L Y A S Y V Y L S M S Y Y F D R D D V A L K N F A K Y F L H Q S H E E R E H A E K L M K L Q N Q R G G R I F L Q D I K K P D C D D W E S G L N A M E C A L H L E K N V N Q S L L E L H K L A T D K N D P H L C D F I E T H Y L N E Q V K A I K E L G D H V T N L R K M G A P E S G L A E Y L F D K H T L G D S D N E S
- Accession
- P02794
- Protein length
- Full Length
- Predicted molecular weight
- 21 kDa
- Amino acids
- 1 to 183
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab78877is purified using conventional chromatography techniques.
General info
- Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293).
- Sequence similarities
Belongs to the ferritin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Recombinant Human Ferritin Heavy Chain protein (ab78877)
All lanes: Western blot - Anti-Ferritin Heavy Chain antibody (Anti-Ferritin Heavy Chain antibody ab65080) at 1 µg/mL
Lane 1: Western blot - Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.1 µg
Lane 2: Western blot - Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 30s
SDS-PAGE - Recombinant Human Ferritin Heavy Chain protein (ab78877)
15% SDS-PAGE showing ab78877 at approximately 21kDa (3μg).
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Product protocols
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