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AB78877

Recombinant Human Ferritin Heavy Chain protein (Tag Free)

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(2 Publications)

Recombinant Human Ferritin Heavy Chain protein (Tag Free) is a Human Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

FTH, FTHL6, OK/SW-cl.84, PIG15, FTH1, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein

2 Images
Western blot - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (AB78877)
  • WB

Unknown

Western blot - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (AB78877)

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody (<a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-ab65080'>ab65080</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (ab78877) at 0.1 µg

Lane 2:

Western blot - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (ab78877) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 21 kDa

true

Exposure time: 30s

SDS-PAGE - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (AB78877)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (AB78877)

15% SDS-PAGE showing ab78877 at approximately 21kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

P02794

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab78877 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-ab65080'>ab65080</a>.</p>" } } }
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Sequence info

[{"sequence":"MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES","proteinLength":"Full Length","predictedMolecularWeight":"21 kDa","actualMolecularWeight":null,"aminoAcidEnd":183,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P02794","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab78877is purified using conventional chromatography techniques.

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General info

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed : 9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed : 9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed : 24695223, PubMed : 26436293).

Sequence similarities

Belongs to the ferritin family.

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Product protocols

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Target data

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed : 9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed : 9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed : 24695223, PubMed : 26436293).
See full target information FTH1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:426 PubMed36284090

2022

Ferrostatin-1 alleviates tissue and cell damage in diabetic retinopathy by improving the antioxidant capacity of the Xc-GPX4 system.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhi Shao,Zhouxian Bai,Lirong Zhang,Fengyan Zhang

Annals of the rheumatic diseases 71:943-7 PubMed22228484

2012

Association of ferritin autoantibodies with giant cell arteritis/polymyalgia rheumatica.

Applications

ELISA

Species

Unspecified reactive species

N T Baerlecken,A Linnemann,W L Gross,F Moosig,T R Vazquez-Rodriguez,M A Gonzalez-Gay,J Martin,I Kötter,J C Henes,I Melchers,P Vaith,R E Schmidt,T Witte
View all publications
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Primary Antibodies

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Proteins & Peptides

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SDS-PAGE - Recombinant Human Ferritin Light Chain protein (AB158473)

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