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AB285462

Recombinant human FGF2 protein (Active)

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Recombinant human FGF2 protein (Active) is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS.

View Alternative Names

FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2

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Functional Studies - Recombinant human FGF2 protein (Active) (AB285462)
  • FuncS

Supplier Data

Functional Studies - Recombinant human FGF2 protein (Active) (AB285462)

The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 30 ng/ml.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS

applications

Biologically active

Yes

Biological activity

The ED50, calculated by the dose-dependent proliferation of BALB/c3T3 cells is typically <100ng/ml.

Accession

P09038

Animal free

Yes

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in 5 mM Tris, pH 7.6

Storage buffer

pH: 7.6 Constituents: 0.87% Sodium chloride, 0.06% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 4037 FGF-2/FGF-basic, human recombinant. 4037-50 is the same size as the 50 μg size of ab285462.

Lyophilized FGF2 is best-stored desiccated below 0°C.

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"17.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P09038","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.
Biological function summary

Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.

Pathways

FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.

Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.
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Specifications

Form

Lyophilized

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General info

Function

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed : 28302677). Binds to integrin ITGAV : ITGB3 (PubMed : 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed : 28302677, PubMed : 8663044). Functions as a potent mitogen in vitro (PubMed : 1721615, PubMed : 3732516, PubMed : 3964259). Can induce angiogenesis (PubMed : 23469107, PubMed : 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed : 29501879).

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational modifications

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed : 28302677). Binds to integrin ITGAV : ITGB3 (PubMed : 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed : 28302677, PubMed : 8663044). Functions as a potent mitogen in vitro (PubMed : 1721615, PubMed : 3732516, PubMed : 3964259). Can induce angiogenesis (PubMed : 23469107, PubMed : 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed : 29501879).
See full target information FGF2
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