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Recombinant human FGF2 protein (Active) is a Human Full Length protein, in the 135 to 288 aa range, expressed in Escherichia coli, with >=95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, CellDiff, CellAct.
Alternative names=Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2, FGF2, FGFB
- Purity
>=95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, HPLC, CellDiff, CellAct
- Biologically active
Yes
- Sequence
A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application CellDiff | Reactivity Reacts | Dilution info - | Notes - |
Application CellAct | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
Recommended products
Recombinant human FGF2 protein (Active) is a Human Full Length protein, in the 135 to 288 aa range, expressed in Escherichia coli, with >=95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, CellDiff, CellAct.
Alternative names=Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2, FGF2, FGFB
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, HPLC, CellDiff, CellAct
- Biologically active
Yes
- Biological activity
Determined by a cell proliferation assay using Balb/c 3T3 cells.
The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Predicted molecular weight
17.2 kDa
- Amino acids
135 to 288
- Sequence
A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S
- Accession
- P09038-4
- Protein length
Full Length
- Animal free
No
- Carrier free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute in 5mM Tris, pH 7.6, to 0.1-1.0 mg/ml.
- Concentration
- Storage buffer
Constituents: 0.87% Sodium chloride, 0.0605% Tris
Specifications
- Form
Lyophilized
- Additional notes
>= 95% HPLCSterile filtered
General info
- Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
- Sequence similarities
Belongs to the heparin-binding growth factors family.
- Post-translational modifications
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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