Recombinant human FGF2 protein (Active) is a Human Full Length protein in the 135 to 288 aa range with ≥95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC and cell culture assays.The predicted molecular weight of ab9596 protein is 17.2 kDa.
- Save time and ensure accurate results - use our recombinant human FGF-2/bFGF/HBGF-2 protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
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Publications
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- Communications biology 5:13492022Controlled release of growth factors using synthetic glycosaminoglycans in a modular macroporous scaffold for tissue regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZ Söderlund,A Ibáñez-Fonseca,S Hajizadeh,J C Rodríguez-Cabello,J Liu,L Ye,E Tykesson,L Elowsson,G Westergren-ThorssonPubMed 36482075
- Frontiers in pharmacology 13:9050822022Posaconazole inhibits the stemness of cancer stem-like cells by inducing autophagy and suppressing the Wnt/β-catenin/survivin signaling pathway in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Wang,Yinfeng Tan,Hao Jia,Danqi Liu,Rangru LiuPubMed 36034873
- Experimental and molecular pathology 118:1045902020Effect of fibroblast growth factor 2 on degenerative endplate chondrocyte: From anabolism to catabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Song,Hongyang Du,Jie Li,Mingming Wang,Jianhua Wang,Xuezhuang Ju,Weidong MuPubMed 33285208
- Molecular oncology 13:290-3062018Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatheus H Dias,Cecília S Fonseca,Julianna D Zeidler,Layra L Albuquerque,Marcelo S da Silva,Eduardo Cararo-Lopes,Marcelo S Reis,Vincent Noël,Edmilson O Dos Santos,Ian A Prior,Hugo A ArmelinPubMed 30422399
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, CellDiff, CellAct
- Biologically active
- Yes
Amino acid sequence
A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application CellDiff | Reactivity Reacts | Dilution info - | Notes - |
Application CellAct | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
Alternative names
FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2
Recommended products
Recombinant human FGF2 protein (Active) is a Human Full Length protein in the 135 to 288 aa range with ≥95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC and cell culture assays.The predicted molecular weight of ab9596 protein is 17.2 kDa.
- Save time and ensure accurate results - use our recombinant human FGF-2/bFGF/HBGF-2 protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, CellDiff, CellAct
- Biologically active
- Yes
- Biological activity
- Determined by a cell proliferation assay using Balb/c 3T3 cells.
The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Accession
- P09038-4
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in 5 mM Tris, pH 7.6
- Concentration
- Storage buffer
Constituents: 0.87% Sodium chloride, 0.0605% Tris
Sequence info
Amino acid sequence
- A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S
- Accession
- P09038
- Protein length
- Full Length
- Predicted molecular weight
- 17.2 kDa
- Amino acids
- 135 to 288
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>= 95% HPLCSterile filtered
General info
- Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
- Sequence similarities
Belongs to the heparin-binding growth factors family.
- Post-translational modifications
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our bioactive recombinant human FGF-2/bFGF/HBGF-2 protein ab9596 as a control in SDS-PAGE and HPLC.
Can be used in cell culture assays for stem cell use.
Check out our protein gel staining guide for SDS-PAGE here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.
- Biological function summary
Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.
- Pathways
FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.
- Associated diseases and disorders
Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.Cell Differentiation - Recombinant human FGF2 protein (Active) (ab9596)
Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF (Recombinant human VEGF 165A protein (Active) ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (Anti-CD31 antibody ab32457 in green) and CD34 (Anti-CD34 antibody [QBEND/10] ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.
Sandwich ELISA - Recombinant human FGF2 protein (Active) (ab9596)
Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (Anti-FGF2 antibody [EP1735] ab92337) at 0.5ug/ml.
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