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Recombinant human FGF2 protein (Active) is a Human Full Length protein, in the 135 to 288 aa range, expressed in Escherichia coli, with >=95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, CellDiff, CellAct.


Images

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC, CellDiff, CellAct

Biologically active

Yes

Amino acid sequence

A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

CellDiff

Reactivity

Reacts

Dilution info

-

Notes

-

Application

CellAct

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).

Alternative names

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Recombinant human FGF2 protein (Active) is a Human Full Length protein, in the 135 to 288 aa range, expressed in Escherichia coli, with >=95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, CellDiff, CellAct.

Alternative names

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Applications

SDS-PAGE, HPLC, CellDiff, CellAct

Biological activity

Determined by a cell proliferation assay using Balb/c 3T3 cells.

The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Accession
P09038-4
Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in 5mM Tris, pH 7.6, to 0.1-1.0 mg/ml.

Concentration
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Storage buffer

Constituents: 0.87% Sodium chloride, 0.0605% Tris

Sequence info

Amino acid sequence

A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S

Accession

P09038

Protein length

Full Length

Predicted molecular weight

17.2 kDa

Amino acids

135 to 288

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>= 95% HPLCSterile filtered

General info

Function

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational modifications

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

Biological function summary

Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.

Activity summary

FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.

Pathways

FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.

Associated diseases and disorders

Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Cell Differentiation - Recombinant human FGF2 protein (Active) (ab9596), expandable thumbnail
    This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.

    Cell Differentiation - Recombinant human FGF2 protein (Active) (ab9596)

    Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.

  • Sandwich ELISA - Recombinant human FGF2 protein (Active) (ab9596), expandable thumbnail

    Sandwich ELISA - Recombinant human FGF2 protein (Active) (ab9596)

    Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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