Recombinant human FGF2 protein (Animal Free) is a Human Full Length protein, in the 135 to 288 aa range, expressed in Escherichia coli, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>97% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
Alternative names
Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2, FGF2, FGFB
Recommended products
Recombinant human FGF2 protein (Animal Free) is a Human Full Length protein, in the 135 to 288 aa range, expressed in Escherichia coli, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names
Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2, FGF2, FGFB
Key facts
- Purity
>97% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL.
- Accession
- P09038-4
- Animal free
Yes
- Carrier free
Yes
- Species
Human
- Reconstitution
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
- Concentration
- Storage buffer
Constituents: 0.14% Sodium phosphate
Sequence info
Amino acid sequence
A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S
- Accession
- Protein length
Full Length
- Predicted molecular weight
17.2 kDa
- Amino acids
135 to 288
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
- Sequence similarities
Belongs to the heparin-binding growth factors family.
- Post-translational modifications
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.
- Biological function summary
Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.
- Pathways
FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.
- Associated diseases and disorders
Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.
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Cellular Activation - Recombinant human FGF2 protein (Animal Free) (ab179490)
Dose-dependent proliferation of mouse BALB/c 3T3 cells with ab179490. Assays were carried out in triplicate.
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