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AB283483

Recombinant human FGF21 protein (Active)

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Recombinant human FGF21 protein (Active) is a Human Full Length protein, in the 29 to 209 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, Cell Culture, HPLC.

View Alternative Names

UNQ3115/PRO10196, FGF21, Fibroblast growth factor 21, FGF-21

4 Images
Mass Spectrometry - Recombinant human FGF21 protein (Active) (AB283483)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human FGF21 protein (Active) (AB283483)

Mass determination by ESI-TOF.

Predicted MW is 19467.90 Da (+/- 10 Da by ESI-TOF). Observed MW is 19469.16

Functional Studies - Recombinant human FGF21 protein (Active) (AB283483)
  • FuncS

Supplier Data

Functional Studies - Recombinant human FGF21 protein (Active) (AB283483)

Functional analysis of ab283483.

Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.

ED50 for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 103 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3395757-1

HPLC - Recombinant human FGF21 protein (Active) (AB283483)
  • HPLC

Supplier Data

HPLC - Recombinant human FGF21 protein (Active) (AB283483)

HPLC analysis of ab283483

SDS-PAGE - Recombinant human FGF21 protein (Active) (AB283483)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human FGF21 protein (Active) (AB283483)

SDS-page analysis of ab283483

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, FuncS, HPLC, Cell Culture, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.

ED50 for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 103 units/mg.

Accession

Q9NSA1

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS","proteinLength":"Full Length","predictedMolecularWeight":"19.47 kDa","actualMolecularWeight":"19.47 kDa","aminoAcidEnd":209,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9NSA1","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
Biological function summary

FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.

Pathways

FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.

FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.
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Specifications

Form

Lyophilized

Additional notes

>95% by HPLC

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General info

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.

Sequence similarities

Belongs to the heparin-binding growth factors family.

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Product protocols

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Target data

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
See full target information FGF21
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