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Recombinant human FGF21 protein (Active) is a Human Full Length protein, in the 29 to 209 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

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Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biologically active

Yes

Actual molecular weight

19.47 kDa

Sequence

H P I P D S S P L L Q F G G Q V R Q R Y L Y T D D A Q Q T E A H L E I R E D G T V G G A A D Q S P E S L L Q L K A L K P G V I Q I L G V K T S R F L C Q R P D G A L Y G S L H F D P E A C S F R E L L L E D G Y N V Y Q S E A H G L P L H L P G N K S P H R D P A P R G P A R F L P L P G L P P A L P E P P G I L A P Q P P D V G S S D P L S M V G P S Q G R S P S Y A S

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human FGF21 protein (Active) is a Human Full Length protein, in the 29 to 209 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biological activity

Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.

ED50 for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 103 units/mg.

Predicted molecular weight

19.47 kDa

Amino acids

29 to 209

Sequence

H P I P D S S P L L Q F G G Q V R Q R Y L Y T D D A Q Q T E A H L E I R E D G T V G G A A D Q S P E S L L Q L K A L K P G V I Q I L G V K T S R F L C Q R P D G A L Y G S L H F D P E A C S F R E L L L E D G Y N V Y Q S E A H G L P L H L P G N K S P H R D P A P R G P A R F L P L P G L P P A L P E P P G I L A P Q P P D V G S S D P L S M V G P S Q G R S P S Y A S

Accession
Q9NSA1-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

>95% by HPLC

General info

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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