Recombinant human FGF21 protein (Active) is a Human Full Length protein, in the 29 to 209 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.
Images
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
Amino acid sequence
H P I P D S S P L L Q F G G Q V R Q R Y L Y T D D A Q Q T E A H L E I R E D G T V G G A A D Q S P E S L L Q L K A L K P G V I Q I L G V K T S R F L C Q R P D G A L Y G S L H F D P E A C S F R E L L L E D G Y N V Y Q S E A H G L P L H L P G N K S P H R D P A P R G P A R F L P L P G L P P A L P E P P G I L A P Q P P D V G S S D P L S M V G P S Q G R S P S Y A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
Alternative names
UNQ3115/PRO10196, FGF21, Fibroblast growth factor 21, FGF-21
Recommended products
Recombinant human FGF21 protein (Active) is a Human Full Length protein, in the 29 to 209 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.
ED50 for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 103 units/mg.
- Accession
- Q9NSA1-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- H P I P D S S P L L Q F G G Q V R Q R Y L Y T D D A Q Q T E A H L E I R E D G T V G G A A D Q S P E S L L Q L K A L K P G V I Q I L G V K T S R F L C Q R P D G A L Y G S L H F D P E A C S F R E L L L E D G Y N V Y Q S E A H G L P L H L P G N K S P H R D P A P R G P A R F L P L P G L P P A L P E P P G I L A P Q P P D V G S S D P L S M V G P S Q G R S P S Y A S
- Accession
- Q9NSA1
- Protein length
- Full Length
- Predicted molecular weight
- 19.47 kDa
- Actual molecular weight
- 19.47 kDa
- Amino acids
- 29 to 209
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>95% by HPLC
General info
- Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
- Sequence similarities
Belongs to the heparin-binding growth factors family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
- Biological function summary
FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.
- Pathways
FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.
- Associated diseases and disorders
FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.
Product promise
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4 product images
Functional Studies - Recombinant human FGF21 protein (Active) (ab283483)
Functional analysis of ab283483.
Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.
ED50 for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 103 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3395757-1
SDS-PAGE - Recombinant human FGF21 protein (Active) (ab283483)
SDS-page analysis of ab283483
Mass Spectrometry - Recombinant human FGF21 protein (Active) (ab283483)
Mass determination by ESI-TOF.
Predicted MW is 19467.90 Da (+/- 10 Da by ESI-TOF). Observed MW is 19469.16
HPLC - Recombinant human FGF21 protein (Active) (ab283483)
HPLC analysis of ab283483
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