Recombinant human FGF5 protein (Active) is a Human Full Length protein, in the 18 to 268 aa range, expressed in Escherichia coli, with >=95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A W A H G E K R L A P K G Q P G P A A T D R N P I G S S S R Q S S S S A M S S S S A S S S P A A S L G S Q G S G L E Q S S F Q W S P S G R R T G S L Y C R V G I G F H L Q I Y P D G K V N G S H E A N M L S V L E I F A V S Q G I V G I R G V F S N K F L A M S K K G K L H A S A K F T D D C K F R E R F Q E N S Y N T Y A S A I H R T E K T G R E W Y V A L N K R G K A K R G C S P R V K P Q H I S T H F L P R F K Q S E Q P E L S F T V T V P E K K N P P S P I K S K I P L S A P R K N T N S V K Y R L K F R F G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity).
Alternative names
Fibroblast growth factor 5, FGF-5, Heparin-binding growth factor 5, Smag-82, HBGF-5, FGF5
Recommended products
Recombinant human FGF5 protein (Active) is a Human Full Length protein, in the 18 to 268 aa range, expressed in Escherichia coli, with >=95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Determined by NR6R-3T3 proliferation with 1 ug heparin.
ED50 ≤10 ng/ml (≥ 1.0 x 105 units/mg).
- Accession
- P12034-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate
Sequence info
Amino acid sequence
- M A W A H G E K R L A P K G Q P G P A A T D R N P I G S S S R Q S S S S A M S S S S A S S S P A A S L G S Q G S G L E Q S S F Q W S P S G R R T G S L Y C R V G I G F H L Q I Y P D G K V N G S H E A N M L S V L E I F A V S Q G I V G I R G V F S N K F L A M S K K G K L H A S A K F T D D C K F R E R F Q E N S Y N T Y A S A I H R T E K T G R E W Y V A L N K R G K A K R G C S P R V K P Q H I S T H F L P R F K Q S E Q P E L S F T V T V P E K K N P P S P I K S K I P L S A P R K N T N S V K Y R L K F R F G
- Accession
- P12034
- Protein length
- Full Length
- Predicted molecular weight
- 27.7 kDa
- Amino acids
- 18 to 268
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity determined by reducing and non-reducing SDS-PAGE.
General info
- Function
Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity).
- Sequence similarities
Belongs to the heparin-binding growth factors family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF5 also known as fibroblast growth factor 5 is a protein involved in various cellular processes with a molecular mass of approximately 29 kDa. It is part of the FGF family which includes at least 22 members playing roles in cell growth differentiation and regulation. FGF5 expression occurs mainly in hair follicles skeletal muscles and the central nervous system. The protein has been identified in epithelial cells and the stromal compartment surrounding hair follicles.
- Biological function summary
FGF5 functions as a signaling molecule that influences hair growth cycle regulation. It is an important player in the transition of hair follicles from the anagen (growth) phase to the catagen (regression) phase. FGF5 does not act alone but interacts with various cellular receptors particularly FGFR1 and FGFR2 which facilitate its role in hair cycle modulation. It is not typically part of a stable multi-protein complex but works as a soluble factor influencing nearby cell behavior.
- Pathways
FGF5 engages with signaling cascades such as the MAPK/ERK pathway and the PI3K/AKT pathway which are significant for cell proliferation and survival. FGF5 can modulate these pathways by binding to FGFR1 and FGFR2 receptors leading to downstream signaling that affects cell behavior. These pathways illustrate its involvement with several other FGF family members such as FGF7 and FGF10 showcasing a broad effect on cellular dynamics and development.
- Associated diseases and disorders
FGF5 has been linked to conditions such as congenital hypotrichosis and alopecia where abnormalities in hair growth cycles occur. Dysfunctions in FGF5 signaling can lead to prolonged anagen phases or premature catagen transitions. Additionally FGF5 has connections to cancer development due to its role in cell proliferation and differentiation; aberrations in FGF5 activity can contribute to tumorigenesis. Although not directly linked proteins like FGFR1 can mediate these effects increasing the complexity of its involvement in disease progression.
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2 product images
SDS-PAGE - Recombinant human FGF5 protein (Active) (ab219125)
4-20% Tris-Glycine SDS-PAGE analysis of 1 μg ab219125, stained with Coomasie blue.
Lane 1: Non-reducing conditions.
Lane 2: Reducing conditions.
Functional Studies - Recombinant human FGF5 protein (Active) (ab219125)
Proliferation of NR6R-3T3 cells induced by ab219125.
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