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AB315081

Recombinant Human FGF9/GAF Protein

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Recombinant Human FGF9/GAF Protein is a Human Full Length protein, in the 4 to 208 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for Mass Spec, HPLC.

View Alternative Names

Fibroblast growth factor 9, FGF-9, Glia-activating factor, Heparin-binding growth factor 9, GAF, HBGF-9, FGF9

2 Images
Mass Spectrometry - Recombinant Human FGF9/GAF Protein (AB315081)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Human FGF9/GAF Protein (AB315081)

Mass determination by ESI-TOF. Predicted MW is 23200 Da (+/- 10 Da by ESI-TOF). Observed MW is 23200.

HPLC - Recombinant Human FGF9/GAF Protein (AB315081)
  • HPLC

Lab

HPLC - Recombinant Human FGF9/GAF Protein (AB315081)

HPLC analysis of ab315081

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC

applications

Biologically active

No

Mass Spectrometry

LC-MS/MS

Accession

P31371

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"LGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS","proteinLength":"Full Length","predictedMolecularWeight":"23.2 kDa","actualMolecularWeight":"23.2 kDa","aminoAcidEnd":208,"aminoAcidStart":4,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P31371","tags":[]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
Biological function summary

FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.

Pathways

The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.

Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.

Specifications

Form

Lyophilized

General info

Function

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational modifications

Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.. N-glycosylated.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
See full target information Fibroblast growth factor 9

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