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AB50034

Recombinant human FGF9/GAF protein

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(2 Publications)

Recombinant human FGF9/GAF protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Fibroblast growth factor 9, FGF-9, Glia-activating factor, Heparin-binding growth factor 9, GAF, HBGF-9, FGF9

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Determined by its ability to stimulate the proliferation of mouse balb/c 3T3 cells using a concentration range of 1.0-5.0 ng/ml.

Accession

P31371

Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.2-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Repeated freeze thaw cycles will result in some loss of activity.

Storage buffer

Constituents: 1.45% Sodium chloride, 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as FGF9.
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Sequence info

[{"sequence":"MAPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P31371","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
Biological function summary

FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.

Pathways

The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.

Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.
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Specifications

Form

Lyophilized

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General info

Function

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational modifications

Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.. N-glycosylated.

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Product protocols

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Target data

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
See full target information Fibroblast growth factor 9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 20:e1011510 PubMed39666736

2024

The Tumor-Associated Calcium Signal Transducer 2 (TACSTD2) oncogene is upregulated in cystic epithelial cells revealing a potential new target for polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Abigail O Smith,William Tyler Frantz,Kenley M Preval,Yvonne J K Edwards,Craig J Ceol,Julie A Jonassen,Gregory J Pazour

Biomolecules 12: PubMed36358989

2022

FGF9 Promotes Expression of HAS2 in Palatal Elevation via the Wnt/β-Catenin/TCF7L2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yidan Sun,Xiyu Ying,Ruomei Li,Mengjia Weng,Jiajun Shi,Zhenqi Chen
View all publications
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Product promise

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