Recombinant human FGFR1OP-FGFR1 protein is a Human Fragment protein, in the 429 to 822 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>70% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
V S A D S S A S M N S G V L L V R P S R L S S S G T P M L A G V S E Y E L P E D P R W E L P R D R L V L G K P L G E G C F G Q V V L A E A I G L D K D K P N R V T K V A V K M L K S D A T E K D L S D L I S E M E M M K M I G K H K N I I N L L G A C T Q D G P L Y V I V E Y A S K G N L R E Y L Q A R R P P G L E Y C Y N P S H N P E E Q L S S K D L V S C A Y Q V A R G M E Y L A S K K C I H R D L A A R N V L V T E D N V M K I A D F G L A R D I H H I D Y Y K K T T N G R L P V K W M A P E A L F D R I Y T H Q S D V W S F G V L L W E I F T L G G S P Y P G V P V E E L F K L L K E G H R M D K P S N C T N E L Y M M M R D C W H A V P S Q R P T F K Q L V E D L D R I V A L T S N Q E Y L D L S M P L D Q Y S P S F P D T R S S T C S S G E D S V F S H E P L P E E P C L P R H P A Q L A N G G L K R R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Alternative names
BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, BFGFR, bFGF-R-1, FLT-2
Recommended products
Recombinant human FGFR1OP-FGFR1 protein is a Human Fragment protein, in the 429 to 822 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>70% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Specific activity of ab201397 determined by kinase assay was found to be 350 nmol/ min/ mg
- Accession
- P11362-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% EDTA, 0.001% PMSF
Sequence info
Amino acid sequence 1
V S A D S S A S M N S G V L L V R P S R L S S S G T P M L A G V S E Y E L P E D P R W E L P R D R L V L G K P L G E G C F G Q V V L A E A I G L D K D K P N R V T K V A V K M L K S D A T E K D L S D L I S E M E M M K M I G K H K N I I N L L G A C T Q D G P L Y V I V E Y A S K G N L R E Y L Q A R R P P G L E Y C Y N P S H N P E E Q L S S K D L V S C A Y Q V A R G M E Y L A S K K C I H R D L A A R N V L V T E D N V M K I A D F G L A R D I H H I D Y Y K K T T N G R L P V K W M A P E A L F D R I Y T H Q S D V W S F G V L L W E I F T L G G S P Y P G V P V E E L F K L L K E G H R M D K P S N C T N E L Y M M M R D C W H A V P S Q R P T F K Q L V E D L D R I V A L T S N Q E Y L D L S M P L D Q Y S P S F P D T R S S T C S S G E D S V F S H E P L P E E P C L P R H P A Q L A N G G L K R R
- Accession
- Protein length
Fragment
- Amino acids
429 to 822
- Nature
Recombinant
Amino acid sequence 2
M A A T A A A V V A E E D T E L R D L L V Q T L E N S G V L N R I K A E L R A A V F L A L E E Q E K V E N K T P L V N E S L K K F L N T K D G R L V A S L V A E F L Q F F N L D F T L A V F Q P E T S T L Q G L E G R E N L A R D L G I I E A E G T V G G P L L L E V I R R C Q Q K E K G P T T G E G A L D L S D V H S P P K S P E G K T S A Q T T P S K
- Accession
- Protein length
Fragment
- Predicted molecular weight
108 kDa
- Amino acids
1 to 173
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
- Post-translational modifications
Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FGFR1OP-FGFR1 is a fusion protein resulting from the translocation between the FGFR1 oncogene and the FGFR1OP gene. This fusion is noteworthy in its mechanical role as it forms a constitutively active tyrosine kinase. The FGFR1OP-FGFR1 protein has a molecular mass that varies depending on the fusion breakpoint generally ranging from 100 to 120 kDa. Expressed predominantly in certain hematopoietic cells this fusion oncoprotein continually sends proliferative signals which alter normal cellular functions.
- Biological function summary
FGFR1OP-FGFR1 fusion plays a significant role in driving abnormal cell growth and survival. The fusion protein is an active component within larger protein complexes that facilitate aberrant signaling. By bypassing typical regulatory mechanisms FGFR1OP-FGFR1 fusion protein leads to unchecked cellular division. In addition to maintaining survival pathways it disrupts normal differentiation processes in affected cells.
- Pathways
FGFR1OP-FGFR1 is heavily implicated in the MAPK and PI3K/AKT signaling pathways which are central to cell cycle progression and survival. Key proteins related to these pathways include RAS RAF and AKT proteins which interact to propagate oncogenic signals downstream of the FGFR1OP-FGFR1 fusion. These interactions lead to heightened proliferative responses and resistance to apoptosis steering cells towards oncogenesis.
- Associated diseases and disorders
FGFR1OP-FGFR1 fusion is mostly connected to hematological malignancies mainly myeloproliferative neoplasms such as 8p11 myeloproliferative syndrome. Myelodysplastic syndromes have also shown associations with this protein. The FGFR1OP-FGFR1 fusion protein interacts closely with other oncoproteins like BCR-ABL enhancing its leukemogenic potential. Understanding and targeting this fusion protein is critical for effective therapies for these disorders.
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4 product images
Functional Studies - Recombinant human FGFR1OP-FGFR1 protein (ab201397)
The specific activity of FGFR1OP-FGFR1 (ab201397) was determined to be 80 nmol/min/mg as per activity assay protocol and was equivalent to 400 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human FGFR1OP-FGFR1 protein (ab201397)
SDS PAGE analysis of ab201397
SDS-PAGE - Recombinant human FGFR1OP-FGFR1 protein (ab201397)
SDS PAGE analysis of ab201397
Functional Studies - Recombinant human FGFR1OP-FGFR1 protein (ab201397)
Sample Kinase assay using ab201397.
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