Recombinant human FGFR2 (mutated K526E) protein is a Human Fragment protein, in the 285 to 821 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
Q P H I Q W I K H V E K N G S K Y G P D G L P Y L K V L K A A G V N T T D K E I E V L Y I R N V T F E D A G E Y T C L A G N S I G I S F H S A W L T V L P A P G R E K E I T A S P D Y L E I A I Y C I G V F L I A C M V V T V I L C R M K N T T K K P D F S S Q P A V H K L T K R I P L R R Q V T V S A E S S S S M N S N T P L V R I T T R L S S T A D T P M L A G V S E Y E L P E D P K W E F P R D K L T L G K P L G E G C F G Q V V M A E A V G I D K D K P K E A V T V A V K M L K D D A T E E D L S D L V S E M E M M K M I G K H K N I I N L L G A C T Q D G P L Y V I V E Y A S K G N L R E Y L R A R R P P G M E Y S Y D I N R V P E E Q M T F K D L V S C T Y Q L A R G M E Y L A S Q K C I H R D L A A R N V L V T E N N V M K I A D F G L A R D I N N I D Y Y K K T T N G R L P V K W M A P E A L F D R V Y T H Q S D V W S F G V L M W E I F T L G G S P Y P G I P V E E L F K L L K E G H R M D K P A N C T N E L Y M M M R D C W H A V P S Q R P T F K Q L V E D L D R I L T L T T N E E Y L D L S Q P L E Q Y S P S Y P D T R S S C S S G D D S V F S P D P M P Y E P C L P Q Y P H I N G S V K T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Specific activity was determined to be 67 nmol/min/mg. |
Associated Products
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
Alternative names
CD332, BEK, KGFR, KSAM, FGFR2, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor
Recommended products
Recombinant human FGFR2 (mutated K526E) protein is a Human Fragment protein, in the 285 to 821 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity, 67 nmol/min/mg.
- Accession
- P21802-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Q P H I Q W I K H V E K N G S K Y G P D G L P Y L K V L K A A G V N T T D K E I E V L Y I R N V T F E D A G E Y T C L A G N S I G I S F H S A W L T V L P A P G R E K E I T A S P D Y L E I A I Y C I G V F L I A C M V V T V I L C R M K N T T K K P D F S S Q P A V H K L T K R I P L R R Q V T V S A E S S S S M N S N T P L V R I T T R L S S T A D T P M L A G V S E Y E L P E D P K W E F P R D K L T L G K P L G E G C F G Q V V M A E A V G I D K D K P K E A V T V A V K M L K D D A T E E D L S D L V S E M E M M K M I G K H K N I I N L L G A C T Q D G P L Y V I V E Y A S K G N L R E Y L R A R R P P G M E Y S Y D I N R V P E E Q M T F K D L V S C T Y Q L A R G M E Y L A S Q K C I H R D L A A R N V L V T E N N V M K I A D F G L A R D I N N I D Y Y K K T T N G R L P V K W M A P E A L F D R V Y T H Q S D V W S F G V L M W E I F T L G G S P Y P G I P V E E L F K L L K E G H R M D K P A N C T N E L Y M M M R D C W H A V P S Q R P T F K Q L V E D L D R I L T L T T N E E Y L D L S Q P L E Q Y S P S Y P D T R S S C S S G D D S V F S P D P M P Y E P C L P Q Y P H I N G S V K T
- Accession
- P21802
- Protein length
- Fragment
- Predicted molecular weight
- 72 kDa
- Amino acids
- 285 to 821
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Assessed by densitometry. Affinity purified.
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
- Post-translational modifications
Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The fibroblast growth factor receptor 2 (FGFR2) also known as CD332 is a protein that functions as a transmembrane receptor tyrosine kinase. FGFR2 is important in signal transduction where it binds to fibroblast growth factors triggering downstream intracellular pathways. FGFR2 has two main isoforms FGFR2b and FGFR2c created through alternative splicing. The molecular weight of FGFR2 varies by isoform typically around 100-110 kDa. It gets expressed in various tissues including epithelial and mesenchymal tissues.
- Biological function summary
FGFR2 plays a role in diverse cellular processes like proliferation differentiation and migration. As part of a receptor-ligand complex FGFR2 partners with fibroblast growth factors to initiate signaling cascades. It contributes to embryonic development tissue repair and angiogenesis. FGFR2b expression predominantly appears in epithelial cells while FGFR2c is found in mesenchymal cells reflecting its distinct role in tissue-specific signaling.
- Pathways
FGFR2 integrates into key signaling networks especially the MAPK and PI3K/AKT pathways. It interacts with proteins like FRS2 and GRB2 which facilitate the activation of downstream signals promoting cell survival and growth. FGFR2's engagement in these pathways is critical for maintaining normal cellular functions and contributes to its involvement in regulating complex cellular responses.
- Associated diseases and disorders
FGFR2 mutations have connections with certain cancers and craniosynostosis syndromes. Alterations in FGFR2 signaling pathways can lead to aberrant cellular growth and have been found in various cancers including breast and gastric cancer. In craniosynostosis syndromes mutations in FGFR2 affect bone development linking it with disorders like Apert syndrome. These conditions demonstrate the pivotal role of FGFR2 in maintaining cellular homeostasis and the consequences of its dysfunction.
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3 product images
Functional Studies - Recombinant human FGFR2 (mutated K526E) protein (ab180049)
The specific activity of FGFR2 (ab180049) was determined to be 67 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human FGFR2 (mutated K526E) protein (ab180049)
SDS PAGE analysis of ab180049
Functional Studies - Recombinant human FGFR2 (mutated K526E) protein (ab180049)
Sample Kinase Activity Plot. ab180049 specific activity was determined to be 67 nmol/min/mg.
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