Recombinant Human FGFR4 Protein (Active)
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Recombinant Human FGFR4 Protein (Active) is a Human Fragment protein, in the 22 to 369 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE, FuncS.
View Alternative Names
CD334, JTK2, TKF, FGFR4, Fibroblast growth factor receptor 4, FGFR-4
- FuncS
Supplier Data
Functional Studies - Recombinant Human FGFR4 Protein (Active) (AB290077)
Fully biologically active determined by its ability to inhibit FGF1-depedent proliferation of Balb/c-3T3 cells. ED50 is ≤ 719.5 ng/ml, corresponding to a specific activity of 1.4 x 10³ units/mg. Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3457041-2.
- FuncS
Supplier Data
Functional Studies - Recombinant Human FGFR4 Protein (Active) (AB290077)
Fully biologically active determined by its ability to inhibit FGF1-depedent proliferation of Balb/c-3T3 cells.
ED50 is ≤ 719.5 ng/ml, corresponding to a specific activity of 1.4 x 103 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3457041-2.
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Human FGFR4 Protein (Active) (AB290077)
Mass determination by ESI-TOF. Predicted MW is 38569.35 Da (+/-10 Da by ESI-TOF). Observed MW is 38574.44 Da.
- HPLC
Supplier Data
HPLC - Recombinant Human FGFR4 Protein (Active) (AB290077)
HPLC analysis of ab290077
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human FGFR4 Protein (Active) (AB290077)
SDS-PAGE analysis of ab290077
- HPLC
Supplier Data
HPLC - Recombinant Human FGFR4 Protein (Active) (AB290077)
HPLC analysis of ab290077
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human FGFR4 Protein (Active) (AB290077)
SDS-PAGE analysis of ab290077
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FGFR4 influences various developmental and metabolic processes. It is important in limb and skeletal development epithelial cell morphogenesis and muscle fiber regeneration. FGFR4 often partners with heparan sulfate proteoglycans to form a signaling complex which defines the specificity and regulation of fibroblast growth factor signaling. The receptor's interaction with other proteins like FRFR4 and 5b5 further modulates its biological activity.
Pathways
FGFR4 participates in the MAPK and PI3K-Akt signaling pathways both important for regulating cell survival and growth. These pathways allow FGFR4 to connect with several other proteins such as FGFR1 and BLU-554. FGFR4's activation within these pathways helps determine cellular responses to hormonal and mitogenic signals further influencing tissue homeostasis and repair mechanisms.
Specifications
Form
Lyophilized
Additional notes
SDS-PAGE >= 95%
General info
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Post-translational modifications
N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19.. Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated.. Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.
Subcellular localisation
Endosome
Target data
Product promise
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