Recombinant Human FGFR4 Protein (Active) is a Human protein, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE, FuncS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Applications
- HPLC, MS, SDS-PAGE, FuncS
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
Alternative names
CD334, JTK2, TKF, FGFR4, Fibroblast growth factor receptor 4, FGFR-4
Recombinant Human FGFR4 Protein (Active) is a Human protein, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE, FuncS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Applications
- HPLC, MS, SDS-PAGE, FuncS
- Biologically active
- No
- Accession
- P22455-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
- Post-translational modifications
N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19.
- Subcellular localisation
- Endosome
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGFR4 or fibroblast growth factor receptor 4 is a receptor tyrosine kinase weighing approximately 110 kDa. This protein plays a role in cellular processes by binding to fibroblast growth factors. FGFR4 is expressed in many tissues including muscle liver and the developing epidermis. It is known by alternate names like CD334 or FRFR4. This receptor's activation leads to receptor dimerization and autophosphorylation triggering downstream signaling pathways that affect cell proliferation differentiation and migration.
- Biological function summary
FGFR4 influences various developmental and metabolic processes. It is important in limb and skeletal development epithelial cell morphogenesis and muscle fiber regeneration. FGFR4 often partners with heparan sulfate proteoglycans to form a signaling complex which defines the specificity and regulation of fibroblast growth factor signaling. The receptor's interaction with other proteins like FRFR4 and 5b5 further modulates its biological activity.
- Pathways
FGFR4 participates in the MAPK and PI3K-Akt signaling pathways both important for regulating cell survival and growth. These pathways allow FGFR4 to connect with several other proteins such as FGFR1 and BLU-554. FGFR4's activation within these pathways helps determine cellular responses to hormonal and mitogenic signals further influencing tissue homeostasis and repair mechanisms.
- Associated diseases and disorders
FGFR4 is linked to certain cancers including hepatocellular carcinoma and colorectal cancer notable in cell lines like HCT116 and others. Overexpression or mutations in FGFR4 can drive tumor progression promoting uncontrolled growth and metastasis. FGFR4's relation to other key proteins such as FRAF and 19h3 highlights its role in pathogenesis making it a potential target for therapeutic interventions. Studies involving FGFR4 ELISA kits enhance understanding of its role in disease development and progression.
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7 product images
HPLC - Recombinant Human FGFR4 Protein (Active) (ab290077)
HPLC analysis of ab290077
Mass Spectrometry - Recombinant Human FGFR4 Protein (Active) (ab290077)
Mass determination by ESI-TOF. Predicted MW is 38569.35 Da (+/-10 Da by ESI-TOF). Observed MW is 38574.44 Da.
Functional Studies - Recombinant Human FGFR4 Protein (Active) (ab290077)
Fully biologically active determined by its ability to inhibit FGF1-depedent proliferation of Balb/c-3T3 cells.
ED50 is ≤ 719.5 ng/ml, corresponding to a specific activity of 1.4 x 103 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3457041-2.
Functional Studies - Recombinant Human FGFR4 Protein (Active) (ab290077)
Fully biologically active determined by its ability to inhibit FGF1-depedent proliferation of Balb/c-3T3 cells.
ED50 is ≤ 719.5 ng/ml, corresponding to a specific activity of 1.4 x 10³ units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3457041-2.SDS-PAGE - Recombinant Human FGFR4 Protein (Active) (ab290077)
SDS-PAGE analysis of ab290077
HPLC - Recombinant Human FGFR4 Protein (Active) (ab290077)
HPLC analysis of ab290077
SDS-PAGE - Recombinant Human FGFR4 Protein (Active) (ab290077)
SDS-PAGE analysis of ab290077
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