Recombinant Human FGL1 protein (Fc Chimera) is a Human Full Length protein, in the 1 to 312 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M A K V F S F I L V T T A L T M G R E I S A L E D C A Q E Q M R L R A Q V R L L E T R V K Q Q Q V K I K Q L L Q E N E V Q F L D K G D E N T V I D L G S K R Q Y A D C S E I F N D G Y K L S G F Y K I K P L Q S P A E F S V Y C D M S D G G G W T V I Q R R S D G S E N F N R G W K D Y E N G F G N F V Q K H G E Y W L G N K N L H F L T T Q E D Y T L K I D L A D F E K N S R Y A Q Y K N F K V G D E K N F Y E L N I G E Y S G T A G D S L A G N F H P E V Q W W A S H Q R M K F S T W D R D H D N Y E G N C A E E D Q S G W W F N R C H S A N L N G V Y Y S G P Y T A K T D N G I V W Y T W H G W W Y S L K S V V M K I R P N D F I P N V I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed:30580966). Responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed:30580966). Secreted by, and promotes growth of, hepatocytes (PubMed:11470158, PubMed:19880967).
Alternative names
Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1, HPS, HFREP-1, LFIRE-1, HFREP1, FGL1
Recommended products
Recombinant Human FGL1 protein (Fc Chimera) is a Human Full Length protein, in the 1 to 312 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1, HPS, HFREP-1, LFIRE-1, HFREP1, FGL1
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q08830-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.24% Tris
Sequence info
Amino acid sequence
M A K V F S F I L V T T A L T M G R E I S A L E D C A Q E Q M R L R A Q V R L L E T R V K Q Q Q V K I K Q L L Q E N E V Q F L D K G D E N T V I D L G S K R Q Y A D C S E I F N D G Y K L S G F Y K I K P L Q S P A E F S V Y C D M S D G G G W T V I Q R R S D G S E N F N R G W K D Y E N G F G N F V Q K H G E Y W L G N K N L H F L T T Q E D Y T L K I D L A D F E K N S R Y A Q Y K N F K V G D E K N F Y E L N I G E Y S G T A G D S L A G N F H P E V Q W W A S H Q R M K F S T W D R D H D N Y E G N C A E E D Q S G W W F N R C H S A N L N G V Y Y S G P Y T A K T D N G I V W Y T W H G W W Y S L K S V V M K I R P N D F I P N V I
- Accession
- Protein length
Full Length
- Predicted molecular weight
60.4 kDa
- Amino acids
1 to 312
- Nature
Recombinant
- Tags
Fc tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed:30580966). Responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed:30580966). Secreted by, and promotes growth of, hepatocytes (PubMed:11470158, PubMed:19880967).
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fibrinogen-like protein 1 (FGL1) also known as Hepassocin plays a significant role in modulating immune responses. FGL1 is a member of the fibrinogen family with a molecular mass of approximately 31 kDa. This protein is widely expressed in the liver and is also found in other tissues like the pancreas and lungs. Its structural homology to fibrinogen suggests potential binding capabilities with similar ligands influencing its mechanical actions in cellular processes.
- Biological function summary
FGL1 modulates the immune system and maintains its homeostasis. It functions primarily as a ligand for the immune checkpoint receptor LAG-3. This FGL1-LAG-3 interaction inhibits T-cell activity leading to immune tolerance and helping prevent autoimmunity. FGL1 while not a part of a complex demonstrates significant standalone biological activity by affecting immune responses especially under pathological conditions.
- Pathways
FGL1 integrates into the immune regulatory framework. It exerts its effects mainly through the immune checkpoint pathway impacting T-cell regulation. In addition to LAG-3 FGL1 interacts closely with the PD-1/PD-L1 pathway which is also important in checkpoint inhibition and immune evasion mechanisms. These pathways together orchestrate the immune surveillance system and play roles in maintaining immune equilibrium.
- Associated diseases and disorders
FGL1 has received attention in cancer particularly in its role of suppressing anti-tumor immunity. Its interaction with LAG-3 makes it a potential target for cancer immunotherapy as high FGL1 levels correlate with poor prognosis in various cancers. Additionally it has implications in autoimmune diseases where its regulation of T-cell activity prevents excessive immune responses. By modulating LAG-3 activity FGL1 emerges as a therapeutic target of interest in autoimmune conditions presenting new opportunities for managing such disorders.
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1 product image
SDS-PAGE - Recombinant Human FGL1 protein (Fc Chimera) (ab276547)
SDS-PAGE analysis of ab276547
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