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AB60854

Recombinant human FGR protein

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Recombinant human FGR protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

SRC2, FGR, Tyrosine-protein kinase Fgr, Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, Proto-oncogene c-Fgr, p55-Fgr, p58-Fgr, p58c-Fgr

4 Images
Functional Studies - Recombinant human FGR protein (AB60854)
  • FuncS

Unknown

Functional Studies - Recombinant human FGR protein (AB60854)

The specific activity of FGR (ab60854) was determined to be 155 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human FGR protein (AB60854)
  • FuncS

Unknown

Functional Studies - Recombinant human FGR protein (AB60854)

Sample Kinase Activity Plot.

SDS-PAGE - Recombinant human FGR protein (AB60854)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human FGR protein (AB60854)

ab60854 on SDS-PAGE, MW ~86 kDa.

SDS-PAGE - Recombinant human FGR protein (AB60854)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human FGR protein (AB60854)

SDS PAGE analysis of ab60854

Key facts

Purity

>95% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

P09769

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

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Complementary Products

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

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Proteins & Peptides

AB105909

Recombinant human FAK protein

Functional Studies - Recombinant human FAK protein (AB105909)

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Cell Lines & Lysates

AB94154

FGR overexpression 293T lysate (whole cell)

Western blot - FGR overexpression 293T lysate (whole cell) (AB94154)

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Primary Antibodies

AB133349

Anti-FGR antibody [EPR3171]

RabMAb|Recombinant

Western blot - Anti-FGR antibody [EPR3171] (AB133349)

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Proteins & Peptides

AB165771

Recombinant Human LONRF2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human LONRF2 protein (GST tag N-Terminus) (AB165771)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P09769","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FGR protein also known as Src-like adapter protein or FGR proto-oncogene is an important cellular component with a mass of approximately 58 kDa. Expressed mainly in hematopoietic cells like macrophages and neutrophils the FGR protein belongs to the Src family of non-receptor tyrosine kinases. This protein has the ability to modulate various cellular processes through its kinase activity influencing the phosphorylation of substrates involved in signal transduction.
Biological function summary

The FGR protein impacts processes related to immune responses and cell communication. It participates in the formation of multi-protein complexes enabling interactions that mediate pathways important for cellular signaling. Through these interactions the FGR protein controls important processes like cell growth differentiation and movement especially in immune cell types. Its role in signal transduction renders it a critical player in various immune functions.

Pathways

The FGR protein integrates into the signaling networks such as the MAPK/ERK pathway and PI3K/AKT pathway which are pivotal for cell survival and proliferation. Within these pathways FGR interacts with other proteins like the GRB2 and PI3K to mediate downstream effects that influence many cellular responses. These interactions ensure that alterations in external signals lead to appropriate cellular outcomes sustaining a balance in cellular behavior and immune functions.

Alterations in the expression or mutation of the FGR protein have associations with certain cancers and inflammatory diseases. Enhanced FGR activity has been observed in specific leukemia cases where its interaction with proteins like ABL1 and BCR-ABL1 contributes to oncogenic processes. Similarly abnormalities in FGR signaling pathways can result in immune dysfunctions implicating proteins like Lyn and Hck in pathologies such as rheumatoid arthritis. Understanding the role of FGR within disease contexts aids in identifying therapeutic targets for these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as a negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as a positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Post-translational modifications

Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling; this does not lead to degradation.. Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as a negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as a positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production (By similarity).
See full target information FGR
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