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AB176074

Recombinant Human FHL3 protein

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Recombinant Human FHL3 protein is a Human Full Length protein, in the 1 to 280 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SLIM2, FHL3, Four and a half LIM domains protein 3, FHL-3, Skeletal muscle LIM-protein 2, SLIM-2

1 Images
SDS-PAGE - Recombinant Human FHL3 protein (AB176074)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human FHL3 protein (AB176074)

15% SDS-PAGE analysis of ab176074 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q13643

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP","proteinLength":"Full Length","predictedMolecularWeight":"33.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":280,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13643","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FHL3 also known as Four and a Half LIM Domains Protein 3 functions as a transcriptional cofactor. It weighs approximately 30 kDa. FHL3 is expressed in skeletal muscle cardiac muscle and to some degree in other tissues such as the spleen and lung. This protein consists of LIM domains known for mediating important protein-protein interactions facilitating its role in cellular activities.
Biological function summary

The target plays an important role in transcriptional regulation and muscle development. FHL3 serves as part of transcriptional complexes and has been shown to interact with muscle-specific transcription factors. It promotes the correct expression of genes involved in the growth and maintenance of muscle tissues. Through these interactions FHL3 influences the structural and functional integrity of muscle cells.

Pathways

FHL3 operates within the Wnt signaling and the insulin-like growth factor (IGF) pathways. In these pathways it interacts with several proteins such as transcription factors like MyoD and Myogenin which are critical for muscle differentiation and regeneration. These interactions help in transmitting signals that modulate gene expression patterns essential for muscle function and repair.

Mutations or aberrant expression of FHL3 associates with muscular dystrophy and certain heart conditions. In muscular dystrophy alterations in FHL3 affect muscle fiber integrity often through its interactions with other myotonic dystrophy proteins. In cardiac conditions FHL3 involvement impacts the heart muscle structure and function through its connection with proteins involved in cardiac remodeling and stress response such as the protein titin.
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Specifications

Form

Liquid

Additional notes

ab176074 is purified using conventional chromatography techniques.

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General info

Function

Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.
See full target information FHL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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