Recombinant Human Fibrillarin protein is a Human Fragment protein, in the 83 to 321 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H R S M G K N V M V E P H R H E G V F I C R G K E D A L V T K N L V P G E S V Y G E K R V S I S E G D D K I E Y R A W N P F R S K L A A A I L G G V D Q I H I K P G A K V L Y L G A A S G T T V S H V S D I V G P D G L V Y A V E F S H R S G R D L I N L A K K R T N I I P V I E D A R H P H K Y R M L I A M V D V I F A D V A Q P D Q T R I V A L N A H T F L R N G G H F V I S I K A N C I D S T A S A E A V F A S E V K K M Q Q E N M K P Q E Q L T L E P Y E R D H A V V V G V Y R P P P K V K N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin
Recommended products
Recombinant Human Fibrillarin protein is a Human Fragment protein, in the 83 to 321 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P22087-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H R S M G K N V M V E P H R H E G V F I C R G K E D A L V T K N L V P G E S V Y G E K R V S I S E G D D K I E Y R A W N P F R S K L A A A I L G G V D Q I H I K P G A K V L Y L G A A S G T T V S H V S D I V G P D G L V Y A V E F S H R S G R D L I N L A K K R T N I I P V I E D A R H P H K Y R M L I A M V D V I F A D V A Q P D Q T R I V A L N A H T F L R N G G H F V I S I K A N C I D S T A S A E A V F A S E V K K M Q Q E N M K P Q E Q L T L E P Y E R D H A V V V G V Y R P P P K V K N
- Accession
- P22087
- Protein length
- Fragment
- Predicted molecular weight
- 28.9 kDa
- Amino acids
- 83 to 321
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99443 is purified using conventional chromatography techniques.
General info
- Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
- Sequence similarities
Belongs to the methyltransferase superfamily. Fibrillarin family.
- Post-translational modifications
Ubiquitinated. Ubiquitination leads to proteasomal degradation (PubMed:19208757). Deubiquitinated by USP36 (PubMed:19208757).
- Subcellular localisation
- Nucleus, Nucleolus, Nucleoplasm
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Fibrillarin protein (ab99443)
15% SDS-PAGE showing ab99443 at approximately 28.9kDa (3μg).
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