Recombinant Human Fibrinogen gamma chain protein is a Human Full Length protein, in the 27 to 437 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S Y V A T R D N C C I L D E R F G S Y C P T T C G I A D F L S T Y Q T K V D K D L Q S L E D I L H Q V E N K T S E V K Q L I K A I Q L T Y N P D E S S K P N M I D A A T L K S R K M L E E I M K Y E A S I L T H D S S I R Y L Q E I Y N S N N Q K I V N L K E K V A Q L E A Q C Q E P C K D T V Q I H D I T G K D C Q D I A N K G A K Q S G L Y F I K P L K A N Q Q F L V Y C E I D G S G N G W T V F Q K R L D G S V D F K K N W I Q Y K E G F G H L S P T G T T E F W L G N E K I H L I S T Q S A I P Y A L R V E L E D W N G R T S T A D Y A M F K V G P E A D K Y R L T Y A Y F A G G D A G D A F D G F D F G D D P S D K F F T S H N G M Q F S T W D N D N D K F E G N C A E Q D G S G W W M N K C H A G H L N G V Y Y Q G G T Y S K A S T P N G Y D N G I I W A T W K T R W Y S M K K T T M K I I P F N R L T I G E G Q Q H H L G G A K Q A G D V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Alternative names
PRO2061, FGG, Fibrinogen gamma chain
Recommended products
Recombinant Human Fibrinogen gamma chain protein is a Human Full Length protein, in the 27 to 437 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P02679-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S Y V A T R D N C C I L D E R F G S Y C P T T C G I A D F L S T Y Q T K V D K D L Q S L E D I L H Q V E N K T S E V K Q L I K A I Q L T Y N P D E S S K P N M I D A A T L K S R K M L E E I M K Y E A S I L T H D S S I R Y L Q E I Y N S N N Q K I V N L K E K V A Q L E A Q C Q E P C K D T V Q I H D I T G K D C Q D I A N K G A K Q S G L Y F I K P L K A N Q Q F L V Y C E I D G S G N G W T V F Q K R L D G S V D F K K N W I Q Y K E G F G H L S P T G T T E F W L G N E K I H L I S T Q S A I P Y A L R V E L E D W N G R T S T A D Y A M F K V G P E A D K Y R L T Y A Y F A G G D A G D A F D G F D F G D D P S D K F F T S H N G M Q F S T W D N D N D K F E G N C A E Q D G S G W W M N K C H A G H L N G V Y Y Q G G T Y S K A S T P N G Y D N G I I W A T W K T R W Y S M K K T T M K I I P F N R L T I G E G Q Q H H L G G A K Q A G D V
- Accession
- P02679
- Protein length
- Full Length
- Predicted molecular weight
- 48.9 kDa
- Amino acids
- 27 to 437
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202200 was purified using conventional chromatography techniques.
General info
- Function
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
- Post-translational modifications
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Fibrinogen gamma chain protein (ab202200)
15% SDS-PAGE analysis of ab202200 (3 μg).
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