Recombinant Human Fibronectin protein is a Human Fragment protein, in the 2177 to 2386 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC
- Biologically active
- No
Amino acid sequence
I G H I P R E D V D Y H L Y P H G P G L N P N A S T G Q E A L S Q T T I S W A P F Q D T S E Y I I S C H P V G T D E E P L Q F R V P G T S T S A T L T G L T R G A T Y N V I V E A L K D Q Q R H K V R E E V V T V G N S V N E G L N Q P T D D S C F D P Y T V S H Y A V G D E W E R M S E S G F K L L C Q C L G F G S G H F R C D S S R W C H D N G V N Y K I G E K W D R Q G E N G Q M M S C T C L G N G K G E F K C D P H E A T C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
Recommended products
Recombinant Human Fibronectin protein is a Human Fragment protein, in the 2177 to 2386 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC
- Biologically active
- No
- Accession
- P02751-15
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- I G H I P R E D V D Y H L Y P H G P G L N P N A S T G Q E A L S Q T T I S W A P F Q D T S E Y I I S C H P V G T D E E P L Q F R V P G T S T S A T L T G L T R G A T Y N V I V E A L K D Q Q R H K V R E E V V T V G N S V N E G L N Q P T D D S C F D P Y T V S H Y A V G D E W E R M S E S G F K L L C Q C L G F G S G H F R C D S S R W C H D N G V N Y K I G E K W D R Q G E N G Q M M S C T C L G N G K G E F K C D P H E A T C
- Accession
- P02751
- Protein length
- Fragment
- Predicted molecular weight
- 23.28 kDa
- Actual molecular weight
- 23.28 kDa
- Amino acids
- 2177 to 2386
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
- Post-translational modifications
Sulfated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human Fibronectin protein (ab283423)
SDS-PAGE analysis of ab283423.
Mass Spectrometry - Recombinant Human Fibronectin protein (ab283423)
Mass determination by ESI-TOF.
Predicted MW is 23280.62 (+/- 10Da by ESI-TOF). MW observed is 23281.76.
HPLC - Recombinant Human Fibronectin protein (ab283423)
HPLC analysis of ab283423.
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