Recombinant Human Fibronectin type III EDB protein
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(1 Publication)
Recombinant Human Fibronectin type III EDB protein is a Human Fragment protein, in the 1244 to 1377 aa range with >90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab209886 protein is 17.6 kDa.
- Save time and ensure accurate results - use our fibronectin type III (FN3) EDB protein as a control
View Alternative Names
FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Fibronectin type III EDB protein (AB209886)
SDS PAGE analysis of ab209886, using BSA as a reference control protein. 4-20% gradient gel was used.
Lane 1 : 10μg BSA
Lane 2 : 5μg BSA
Lane 3 : 1μg BSA
Lane 4 : 10μl ab209886
Lane 5 : 5μl ab209886
Lane 6 : 1μl ab209886
Reactivity data
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
The FN3 EDB ab209886 was expressed in E.coli as inclusion bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology and chromatographically purified.
Ensure the validity of your result using our recombinant human fibronectin type III (FN3) EDB protein ab155639 as a positive control in SDS-PAGE.
ab155639 can be used for the following assays:
1. In vitro FN-III EDB domain mediated human endothelial cell or cancer stroma cell differentiation / migration regulations study with this protein as either soluble factor or coating matrix protein.
2. In vitro protein-protein interaction mapping.
Function
Extracellular matrix protein fibronectin (FN) plays an important role in cell adhesion. FN contains FN type I, II and III domains. The FN III repeat is generally about 90 amino acid long and to be composed of seven b-strands, forming two antiparallel b-sheets. In the C-terminal of human fibronectin region, there are 16 FN-III repeat domains. FN III domain derived proteins have been demonstrated to bind various proteins with high affinity.
The alternatively-spliced extra-domain B (EDB) of fibronectin represents one of the best characterized markers of angiogenesis. This domain of 91-aminoacids is completely identical between mouse and man, is virtually absent in normal adult tissues (exception made for the endometrium in the proliferative phase and some vessels of the ovaries), but is strongly expressed in most aggressive solid cancer types, with a prominent vascular and/or stromal pattern of expression.
Check out our protein gel staining guide for SDS-PAGE here
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Specifications
Form
Liquid
Additional notes
ab209886 was sterile-filtered.
General info
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
Post-translational modifications
Sulfated.. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated.. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).. Phosphorylated by FAM20C in the extracellular medium.. Proteolytic processing produces the C-terminal NC1 peptide, anastellin.. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.. Serotonylated on Gln residues by TGM2 in response to hypoxia.
Product protocols
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Target data
Publications (1)
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Nature communications 13:6178 PubMed36261433
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Associated Products
Alternative Product
Proteins & Peptides
AB92784
Native Mouse Fibronectin protein
proteins-peptides
native-mouse-fibronectin-protein-ab92784
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