Recombinant Human Fibronectin type III EDB protein is a Human Fragment protein, in the 1244 to 1377 aa range with >90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab209886 protein is 17.6 kDa.
- Save time and ensure accurate results - use our fibronectin type III (FN3) EDB protein as a control
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Publications
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- Nature communications 13:61782022Helical ultrastructure of the metalloprotease meprin α in complex with a small molecule inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles Bayly-Jones,Christopher J Lupton,Claudia Fritz,Hariprasad Venugopal,Daniel Ramsbeck,Michael Wermann,Christian Jäger,Alex de Marco,Stephan Schilling,Dagmar Schlenzig,James C WhisstockPubMed 36261433
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His-T7 tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
Amino acid sequence
M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F N V S V Y T V K D D K E S V P I S D T I I P E V P Q L T D L S F V D I T D S S I G L R W T P L N S S T I I G Y R I T V V A A G E G I P I F E D F V D S S V G Y Y T V T G L E P G I D Y D I S V I T L I N G G E S A P T T L T Q Q T A V P P P T D L R F T N I G P D T M R V T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes This antibody may be used for in vitro FN-III EDB domain mediated human endothelial cell or cancer stroma cell differentiation / migration regulations study with this protein as either soluble factor or coating matrix protein, for in vitro protein-protein interaction mapping and as antigen for specific antibody production. |
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Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
Recommended products
Recombinant Human Fibronectin type III EDB protein is a Human Fragment protein, in the 1244 to 1377 aa range with >90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab209886 protein is 17.6 kDa.
- Save time and ensure accurate results - use our fibronectin type III (FN3) EDB protein as a control
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His-T7 tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
- Accession
- P02751-7
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.32% Tris HCl
Sequence info
Amino acid sequence
- M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F N V S V Y T V K D D K E S V P I S D T I I P E V P Q L T D L S F V D I T D S S I G L R W T P L N S S T I I G Y R I T V V A A G E G I P I F E D F V D S S V G Y Y T V T G L E P G I D Y D I S V I T L I N G G E S A P T T L T Q Q T A V P P P T D L R F T N I G P D T M R V T
- Accession
- P02751
- Protein length
- Fragment
- Predicted molecular weight
- 17.62 kDa
- Amino acids
- 1244 to 1377
- Nature
- Recombinant
- Tags
- His-T7 tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab209886 was sterile-filtered.
General info
- Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
- Post-translational modifications
Sulfated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Notes
The FN3 EDB ab209886 was expressed in E.coli as inclusion bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology and chromatographically purified.
Ensure the validity of your result using our recombinant human fibronectin type III (FN3) EDB protein Recombinant human EGFR protein (Active) ab155639 as a positive control in SDS-PAGE.
Recombinant human EGFR protein (Active) ab155639 can be used for the following assays:
1. In vitro FN-III EDB domain mediated human endothelial cell or cancer stroma cell differentiation / migration regulations study with this protein as either soluble factor or coating matrix protein.
2. In vitro protein-protein interaction mapping.
Function
Extracellular matrix protein fibronectin (FN) plays an important role in cell adhesion. FN contains FN type I, II and III domains. The FN III repeat is generally about 90 amino acid long and to be composed of seven b-strands, forming two antiparallel b-sheets. In the C-terminal of human fibronectin region, there are 16 FN-III repeat domains. FN III domain derived proteins have been demonstrated to bind various proteins with high affinity.
The alternatively-spliced extra-domain B (EDB) of fibronectin represents one of the best characterized markers of angiogenesis. This domain of 91-aminoacids is completely identical between mouse and man, is virtually absent in normal adult tissues (exception made for the endometrium in the proliferative phase and some vessels of the ovaries), but is strongly expressed in most aggressive solid cancer types, with a prominent vascular and/or stromal pattern of expression.
Check out our protein gel staining guide for SDS-PAGE here
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SDS-PAGE - Recombinant Human Fibronectin type III EDB protein (ab209886)
SDS PAGE analysis of ab209886, using BSA as a reference control protein. 4-20% gradient gel was used.
Lane 1: 10μg BSA
Lane 2: 5μg BSA
Lane 3: 1μg BSA
Lane 4: 10μl ab209886
Lane 5: 5μl ab209886
Lane 6: 1μl ab209886
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