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AB316687

Recombinant Human Fibulin 1 Protein Standard (His tag)

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Recombinant Human Fibulin 1 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

PP213, FBLN1, Fibulin-1, FIBL-1

2 Images
Sandwich ELISA - Recombinant Human Fibulin 1 Protein Standard (His tag) (AB316687)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Fibulin 1 Protein Standard (His tag) (AB316687)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Fibulin 1 Protein Standard (His tag) (AB316687)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Fibulin 1 Protein Standard (His tag) (AB316687)

SDS-PAGE analysis of ab316687 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P23142

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Primary Antibodies

AB175204

Anti-Fibulin 1 antibody [EPR10913]

RabMAb|Recombinant

Western blot - Anti-Fibulin 1 antibody [EPR10913] (AB175204)

  • 0
  • 0
Proteins & Peptides

AB114844

Recombinant Human Fibulin 2 protein

SDS-PAGE - Recombinant Human Fibulin 2 protein (AB114844)

  • 0
  • 0
Proteins & Peptides

AB182808

Recombinant Human Fibulin-4 protein

  • 0
  • 0
Primary Antibodies

AB222325

Anti-K80 antibody

Immunocytochemistry/ Immunofluorescence - Anti-K80 antibody (AB222325)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"96.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P23142","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Specifications

Form

Liquid

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General info

Function

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.

Sequence similarities

Belongs to the fibulin family.

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Product protocols

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Target data

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
See full target information FBLN1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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