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AB316520

Recombinant Human Ficolin-2 Protein Standard (His tag)

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Recombinant Human Ficolin-2 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

FCNL, FCN2, Ficolin-2, 37 kDa elastin-binding protein, Collagen/fibrinogen domain-containing protein 2, Ficolin-B, Ficolin-beta, Hucolin, L-ficolin, Serum lectin p35, EBP-37

2 Images
Sandwich ELISA - Recombinant Human Ficolin-2 Protein Standard (His tag) (AB316520)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Ficolin-2 Protein Standard (His tag) (AB316520)

Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.

SDS-PAGE - Recombinant Human Ficolin-2 Protein Standard (His tag) (AB316520)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Ficolin-2 Protein Standard (His tag) (AB316520)

SDS-PAGE analysis of ab316520 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q15485

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Proteins & Peptides

AB151947

Recombinant Human Mannan Binding Lectin/MBL protein

  • 0
  • 0
Primary Antibodies

AB190834

Anti-MBL1+MBL2 antibody [EPR18381-156]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBL1+MBL2 antibody [EPR18381-156] (AB190834)

  • 0
  • 0
Proteins & Peptides

AB276340

Recombinant Human FCN1/M-Ficolin protein (His tag)

SDS-PAGE - Recombinant Human FCN1/M-Ficolin protein (His tag) (AB276340)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"26.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15485","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 10679061, PubMed : 14707097, PubMed : 15804047, PubMed : 17215869, PubMed : 25344472, PubMed : 8576206). Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed : 14707097). Specifically binds N-Acetylglucosamine (GlcNAc), as well as phosphocholine and lipoteichoic acid moieties on the surface of pathogens (PubMed : 14707097, PubMed : 17215869, PubMed : 25344472, PubMed : 8576206). Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect (PubMed : 8576206).

Sequence similarities

Belongs to the ficolin lectin family.

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Product protocols

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Target data

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 10679061, PubMed : 14707097, PubMed : 15804047, PubMed : 17215869, PubMed : 25344472, PubMed : 8576206). Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed : 14707097). Specifically binds N-Acetylglucosamine (GlcNAc), as well as phosphocholine and lipoteichoic acid moieties on the surface of pathogens (PubMed : 14707097, PubMed : 17215869, PubMed : 25344472, PubMed : 8576206). Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect (PubMed : 8576206).
See full target information FCN2
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Product promise

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