Recombinant Human Filaggrin protein (His tag) is a Human Fragment protein, in the 3838 to 4061 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
D S S R H S Q S G Q G E S A G S R R S R R Q G S S V S Q D S D S E A Y P E D S E R R S E S A S R N H H G S S R E Q S R D G S R H P G S S H R D T A S H V Q S S P V Q S D S S T A K E H G H F S S L S Q D S A Y H S G I Q S R G S P H S S S S Y H Y Q S E G T E R Q K G Q S G L V W R H G S Y G S A D Y D Y G E S G F R H S Q H G S V S Y N S N P V V F K E R S D I C K A S A F G K D H P R Y Y A T Y I N K D P G L C G H S S D I S K Q L G F S Q S Q R Y Y Y Y E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Alternative names
Filaggrin, FLG
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Recombinant Human Filaggrin protein (His tag) is a Human Fragment protein, in the 3838 to 4061 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P20930-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- D S S R H S Q S G Q G E S A G S R R S R R Q G S S V S Q D S D S E A Y P E D S E R R S E S A S R N H H G S S R E Q S R D G S R H P G S S H R D T A S H V Q S S P V Q S D S S T A K E H G H F S S L S Q D S A Y H S G I Q S R G S P H S S S S Y H Y Q S E G T E R Q K G Q S G L V W R H G S Y G S A D Y D Y G E S G F R H S Q H G S V S Y N S N P V V F K E R S D I C K A S A F G K D H P R Y Y A T Y I N K D P G L C G H S S D I S K Q L G F S Q S Q R Y Y Y Y E
- Accession
- P20930
- Protein length
- Fragment
- Predicted molecular weight
- 26.8 kDa
- Amino acids
- 3838 to 4061
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
- Sequence similarities
Belongs to the S100-fused protein family.
- Post-translational modifications
Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Filaggrin protein (His tag) (ab240777)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab240777.
Downloads
Product protocols
- Visit the general protocols
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