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AB240777

Recombinant Human Filaggrin protein (His tag)

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Recombinant Human Filaggrin protein (His tag) is a Human Fragment protein, in the 3838 to 4061 aa range, expressed in Yeast, with >90%, suitable for SDS-PAGE.

View Alternative Names

Filaggrin, FLG

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SDS-PAGE - Recombinant Human Filaggrin protein (His tag) (AB240777)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Filaggrin protein (His tag) (AB240777)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab240777.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

6x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P20930

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE","proteinLength":"Fragment","predictedMolecularWeight":"26.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":4061,"aminoAcidStart":3838,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"P20930","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Sequence similarities

Belongs to the S100-fused protein family.

Post-translational modifications

Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.. Undergoes deimination of some arginine residues (citrullination).

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Product protocols

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Target data

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
See full target information FLG
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Complementary Products

Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

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Multiplex immunohistochemistry - Anti-Filaggrin antibody [EPR21892] (AB221155)

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Assay Kits

AB195215

Human IgG ELISA Kit

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Sandwich ELISA - Human IgG ELISA Kit (AB195215)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB85679

Anti-Loricrin antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody (AB85679)

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Product promise

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