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Recombinant Human FIP protein is a Human Full Length protein, in the 1 to 346 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

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Images

SDS-PAGE - Recombinant Human FIP protein (AB152790), expandable thumbnail

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, ELISA, WB

Biologically active

No

Amino acid sequence

M D M L D P G L D P A A S A T A A A A A S H D K G P E A E E G V E L Q E G G D G P G A E E Q T A V A I T S V Q Q A A F G D H N I Q Y Q F R T E T N G G Q V T Y R V V Q V T D G Q L D G Q G D T A G A V S V V S T A A F A G G Q Q A V T Q V G V D G A A Q R P G P A A A S V P P G P A A P F P L A V I Q N P F S N G G S P A A E A V S G E A R F A Y F P A S S V G D T T A V S V Q T T D Q S L Q A G G Q F Y V M M T P Q D V L Q T G T Q R T I A P R T H P Y S P K I D G T R T P R D E R R R A Q H N E V E R R R R D K I N N W I V Q L S K I I P D C N A D N S K T G A S K G G I L S K A C D Y I R E L R Q T N Q R M Q E T F K E A E R L Q M D N E L L R Q Q I E E L K N E N A L L R A Q L Q Q H N L E M V G E G T R Q

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Alternative names

Recommended products

Recombinant Human FIP protein is a Human Full Length protein, in the 1 to 346 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

Key facts

Expression system

Wheat germ

Applications

SDS-PAGE, ELISA, WB

Accession
Q15853-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M D M L D P G L D P A A S A T A A A A A S H D K G P E A E E G V E L Q E G G D G P G A E E Q T A V A I T S V Q Q A A F G D H N I Q Y Q F R T E T N G G Q V T Y R V V Q V T D G Q L D G Q G D T A G A V S V V S T A A F A G G Q Q A V T Q V G V D G A A Q R P G P A A A S V P P G P A A P F P L A V I Q N P F S N G G S P A A E A V S G E A R F A Y F P A S S V G D T T A V S V Q T T D Q S L Q A G G Q F Y V M M T P Q D V L Q T G T Q R T I A P R T H P Y S P K I D G T R T P R D E R R R A Q H N E V E R R R R D K I N N W I V Q L S K I I P D C N A D N S K T G A S K G G I L S K A C D Y I R E L R Q T N Q R M Q E T F K E A E R L Q M D N E L L R Q Q I E E L K N E N A L L R A Q L Q Q H N L E M V G E G T R Q

Accession

Q15853

Protein length

Full Length

Predicted molecular weight

63.4 kDa

Amino acids

1 to 346

Nature

Recombinant

Specifications

Form

Liquid

General info

Function

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Protein previously labeled as USF2.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FIP also known as Family Interacting Protein or Optineurin is a multifunctional protein with a mass of approximately 66 kDa. It is expressed in various tissues with the highest levels in the heart brain and skeletal muscles. FIP functions mechanically as a scaffold protein interacting with a variety of cellular partners to regulate numerous processes. It contains multiple domains that allow it to bind to other proteins and influence cellular signaling pathways.

Biological function summary

FIP impacts cell signaling vesicle trafficking and inflammation. It often participates as a part of larger protein complexes aiding in the fine-tuning of cellular responses. FIP acts as an important mediator in autophagy assisting in the degradation of damaged cellular components. The protein also modulates the inflammatory response by interacting with signaling molecules impacting pathways like NF-kB signaling and mitigating excessive inflammation.

Pathways

FIP influences cellular homeostasis and immune responses. It plays a significant role in the NF-kB signaling pathway which regulates immune responses and cell survival. FIP forms interactions with proteins such as RIPK1 and ubiquitin chains affecting downstream signaling events. Additionally it participates in the autophagy pathway collaborating with proteins like LC3 to mediate the clearance of cellular debris which is critical for maintaining cellular health.

Associated diseases and disorders

Aberrations in FIP function have been associated with glaucoma and amyotrophic lateral sclerosis (ALS). In glaucoma mutations in FIP are connected to altered autophagic processes impacting optic nerve health. The protein also interacts with TBK1 another factor linked to variations in immune and inflammatory responses contributing to the disease. Furthermore in ALS dysregulation in FIP’s regulatory role in autophagy and inflammation might accelerate neuronal degeneration highlighting its potential as a target for therapeutic intervention.

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1 product image

  • SDS-PAGE - Recombinant Human FIP protein (ab152790), expandable thumbnail

    SDS-PAGE - Recombinant Human FIP protein (ab152790)

    12.5% SDS-PAGE showing ab152790 stained with Coomassie Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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