Recombinant human FIST protein is a Human Fragment protein, in the 163 to 562 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for WB, FuncS.
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Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Alternative names
DYRK6, FIST3, PKY, HIPK3, Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, Fas-interacting serine/threonine-protein kinase, Homolog of protein kinase YAK1, ANPK, FIST
Recommended products
Recombinant human FIST protein is a Human Fragment protein, in the 163 to 562 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for WB, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- WB, FuncS
- Biologically active
- Yes
- Biological activity
- The Specific activity of ab85601 was determined to be 48 nmol/min/mg.
- Accession
- Q9H422-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- Q9H422
- Protein length
- Fragment
- Amino acids
- 163 to 562
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
- Post-translational modifications
Autophosphorylated, but autophosphorylation is not required for catalytic activity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Previously labelled as HIPK3.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FIST protein also known as Found in Squamous Cell Carcinoma Transporter possesses a mass of approximately 60 kDa. It functions mechanically as a transporter protein facilitating the movement of molecules across cellular membranes. Expression of FIST occurs in various tissues with particular abundance in squamous cell carcinoma cells where it plays a role in tumor cell survival and proliferation.
- Biological function summary
FIST contributes to cellular processes by regulating the transport of specific substrates such as ions and small molecules critical for cellular homeostasis and energy balance. This protein is often part of a larger transporter complex allowing it to interact with other proteins to modulate its transport activities efficiently. Its role becomes more significant in squamous cell carcinoma where altered transport activities could contribute to the development and progression of the cancer.
- Pathways
FIST has significant involvement in the cellular energy metabolism and signaling pathways. One key pathway is the ion transport pathway where FIST interacts with several channel proteins to maintain ionic concentration and cellular excitability. Moreover it associates with ATP-binding cassette (ABC) transporters participating in the regulation of multidrug resistance which provides cancer cells with a mechanism to survive chemotherapeutic treatments.
- Associated diseases and disorders
FIST has associations with squamous cell carcinoma and an increased risk of resistance to cancer therapies. Alterations in FIST expression or function can disrupt cellular ion homeostasis contributing to tumor development and progression. The protein links to the disease through its interaction with channel proteins affecting cell proliferation. Additionally its collaboration with ABC transporters in multidrug resistance pathways impacts the effectiveness of chemotherapy making the study of FIST critical in understanding and combating squamous cell carcinoma.
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2 product images
Functional Studies - Recombinant human FIST protein (ab85601)
The Specific activity of ab85601 was determined to be 48 nmol/min/mg.
SDS-PAGE - Recombinant human FIST protein (ab85601)
SDS-PAGE showing ab85601 at approximately 49kDa.
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