Recombinant Human FKBP51 protein (His tag) is a Human Full Length protein, in the 1 to 457 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M K H H H H H H A S M T T D E G A K N N E E S P T A T V A E Q G E D I T S K K D R G V L K I V K R V G N G E E T P M I G D K V Y V H Y K G K L S N G K K F D S S H D R N E P F V F S L G K G Q V I K A W D I G V A T M K K G E I C H L L C K P E Y A Y G S A G S L P K I P S N A T L F F E I E L L D F K G E D L F E D G G I I R R T K R K G E G Y S N P N E G A T V E I H L E G R C G G R M F D C R D V A F T V G E G E D H D I P I G I D K A L E K M Q R E E Q C I L Y L G P R Y G F G E A G K P K F G I E P N A E L I Y E V T L K S F E K A K E S W E M D T K E K L E Q A A I V K E K G T V Y F K G G K Y M Q A V I Q Y G K I V S W L E M E Y G L S E K E S K A S E S F L L A A F L N L A M C Y L K L R E Y T K A V E C C D K A L G L D S A N E K G L Y R R G E A Q L L M N E F E S A K G D F E K V L E V N P Q N K A A R L Q I S M C Q K K A K E H N E R D R R I Y A N M F K K F A E Q D A K E E A N K A M G K K T S E G V T N E K G T D S Q A M E E E K P E G H V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277).
Alternative names
Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54, FKBP5, FKBP51, AIG6
Recommended products
Recombinant Human FKBP51 protein (His tag) is a Human Full Length protein, in the 1 to 457 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, MS.
Alternative names
Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54, FKBP5, FKBP51, AIG6
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- Q13451-1
- Animal free
No
- Species
Human
- Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
- Concentration
- Storage buffer
Constituents: 0.32% Tris buffer, 0.29% Sodium chloride
Sequence info
Amino acid sequence
M K H H H H H H A S M T T D E G A K N N E E S P T A T V A E Q G E D I T S K K D R G V L K I V K R V G N G E E T P M I G D K V Y V H Y K G K L S N G K K F D S S H D R N E P F V F S L G K G Q V I K A W D I G V A T M K K G E I C H L L C K P E Y A Y G S A G S L P K I P S N A T L F F E I E L L D F K G E D L F E D G G I I R R T K R K G E G Y S N P N E G A T V E I H L E G R C G G R M F D C R D V A F T V G E G E D H D I P I G I D K A L E K M Q R E E Q C I L Y L G P R Y G F G E A G K P K F G I E P N A E L I Y E V T L K S F E K A K E S W E M D T K E K L E Q A A I V K E K G T V Y F K G G K Y M Q A V I Q Y G K I V S W L E M E Y G L S E K E S K A S E S F L L A A F L N L A M C Y L K L R E Y T K A V E C C D K A L G L D S A N E K G L Y R R G E A Q L L M N E F E S A K G D F E K V L E V N P Q N K A A R L Q I S M C Q K K A K E H N E R D R R I Y A N M F K K F A E Q D A K E E A N K A M G K K T S E G V T N E K G T D S Q A M E E E K P E G H V
- Accession
- Protein length
Full Length
- Predicted molecular weight
52.5 kDa
- Amino acids
1 to 457
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277).
- Post-translational modifications
Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1 (PubMed:28147277). Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation (PubMed:28147277).
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
FKBP51 also known as FKBP5 is an immunophilin protein with a molecular mass around 51 kDa. It functions as a peptidyl-prolyl isomerase that assists protein folding by catalyzing the cis-trans isomerization of prolyl bonds. FKBP51 is expressed in various tissues including the brain liver kidney and skeletal muscle. The protein serves multiple mechanical roles including the modulation of protein receptor complexes and interaction with other molecular chaperones.
- Biological function summary
FKBP51 plays a role in cellular stress responses and steroid receptor signaling. It is part of large complexes such as the glucocorticoid receptor complex where it modulates receptor sensitivity and translocation to the nucleus. FKBP51 influences the stress hormone pathway by affecting the receptor's binding affinity and contributing to the regulation of inflammatory responses. By playing a regulatory role in hormonal signaling FKBP51 impacts several biological processes including development and metabolism.
- Pathways
FKBP51 interacts with the hypothalamus-pituitary-adrenal (HPA) axis and the AKT/mTOR signaling pathway. It interacts with proteins like Hsp90 and PHLPP impacting stress response and cellular growth regulation. The modulation of these pathways affects stress-related behaviors and cellular homeostasis. FKBP51's interaction with the HPA axis plays a significant role in stress response regulation making it an essential component of the body's adaptation mechanisms.
- Associated diseases and disorders
FKBP51 has strong associations with mood disorders and cancer. Its upregulation correlates with stress-related psychiatric conditions such as depression impacting the function of other stress-related proteins like glucocorticoid receptors. In cancer FKBP51 modulates tumor growth and survival pathways interacting with proteins involved in cell proliferation and death such as the AKT protein. Understanding FKBP51's role in these conditions helps identify potential therapeutic targets for treatment.
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1 product image
SDS-PAGE - Recombinant Human FKBP51 protein (His tag) (ab206440)
12 % SDS-PAGE separation of Human FKBP5.
MW Marker: 14, 21, 31, 45, 66, 97 kDa
Lane 1: Reduced and boiled sample, 2.5 μg/lane
Lane 2: Non-reduced and non-boiled sample, 2.5 μg/lane
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