JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314618

Recombinant Human FKTN Protein (His Tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human FKTN Protein (His Tag) is a Human Full Length protein, in the 1 to 461 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

FCMD, FKTN, Ribitol-5-phosphate transferase FKTN, Fukutin, Fukuyama-type congenital muscular dystrophy protein, Ribitol-5-phosphate transferase

1 Images
SDS-PAGE - Recombinant Human FKTN Protein (His Tag) (AB314618)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human FKTN Protein (His Tag) (AB314618)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O75072

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MSRINKNVVLALLTLTSSAFLLFQLYYYKHYLSTKNGAGLSKSKGSRIGFDSTQWRAVKKFIMLTSNQNVPVFLIDPLILELINKNFEQVKNTSHGSTSQCKFFCVPRDFTAFALQYHLWKNEEGWFRIAENMGFQCLKIESKDPRLDGIDSLSGTEIPLHYICKLATHAIHLVVFHERSGNYLWHGHLRLKEHIDRKFVPFRKLQFGRYPGAFDRPELQQVTVDGLEVLIPKDPMHFVEEVPHSRFIECRYKEARAFFQQYLDDNTVEAVAFRKSAKELLQLAAKTLNKLGVPFWLSSGTCLGWYRQCNIIPYSKDVDLGIFIQDYKSDIILAFQDAGLPLKHKFGKVEDSLELSFQGKDDVKLDVFFFYEETDHMWNGGTQAKTGKKFKYLFPKFTLCWTEFVDMKVHVPCETLEYIEANYGKTWKIPVKTWDWKRSPPNVQPNGIWPISEWDEVIQLY","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"56.5 kDa","aminoAcidEnd":461,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75072","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fukutin also known as FKRP or FKTN is a glycosyltransferase enzyme weighing approximately 55 kDa. This protein participates in the process of adding sugar chains to proteins primarily glycosylating α-dystroglycan. Fukutin is highly expressed in muscle tissues including skeletal and cardiac muscles and plays a role in the central nervous system. Its activity is important for the proper function of these tissues.
Biological function summary

Fukutin plays a fundamental role in maintaining the structure and function of muscle cells by participating in the glycosylation of α-dystroglycan. This modification allows α-dystroglycan to bind to extracellular matrix proteins. Fukutin operates as part of the dystrophin-glycoprotein complex which is essential for muscle integrity and stability. This complex anchors the cytoskeleton of a muscle cell to the surrounding extracellular matrix.

Pathways

This protein fukutin participates in the dystroglycan pathway affecting the integrity of muscle cells. It works closely with other proteins like LARGE1 which also modifies α-dystroglycan. Fukutin's activity ensures that α-dystroglycan has the necessary post-translational modifications to perform its function within the laminin-binding pathway linking the extracellular matrix to the cytoskeleton.

Alterations in fukutin mutations can lead to muscular dystrophies such as Fukuyama congenital muscular dystrophy and Walker-Warburg syndrome. In these conditions the fukutin-related glycosylation of α-dystroglycan is disrupted which impairs muscle cell stability and function. Other proteins like dystrophin and sarcoglycans associated with the dystrophin-glycoprotein complex also play roles in these disorders further linking fukutin to muscle disease mechanisms.
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

Function

Catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed : 26923585, PubMed : 27194101, PubMed : 29477842). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (PubMed : 17034757, PubMed : 25279699, PubMed : 26923585, PubMed : 27194101, PubMed : 29477842). Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity (PubMed : 17034757). May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes (PubMed : 25279699). Could be involved in brain development (Probable).

Sequence similarities

Belongs to the LicD transferase family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed : 26923585, PubMed : 27194101, PubMed : 29477842). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (PubMed : 17034757, PubMed : 25279699, PubMed : 26923585, PubMed : 27194101, PubMed : 29477842). Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity (PubMed : 17034757). May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes (PubMed : 25279699). Could be involved in brain development (Probable).
See full target information FKTN
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB131280

Anti-Fukutin antibody [EPR7913]

RabMAb|Recombinant

Western blot - Anti-Fukutin antibody [EPR7913] (AB131280)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com