Recombinant Human FKTN Protein (His Tag) is a Human Full Length protein, in the 1 to 461 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- 10x His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S R I N K N V V L A L L T L T S S A F L L F Q L Y Y Y K H Y L S T K N G A G L S K S K G S R I G F D S T Q W R A V K K F I M L T S N Q N V P V F L I D P L I L E L I N K N F E Q V K N T S H G S T S Q C K F F C V P R D F T A F A L Q Y H L W K N E E G W F R I A E N M G F Q C L K I E S K D P R L D G I D S L S G T E I P L H Y I C K L A T H A I H L V V F H E R S G N Y L W H G H L R L K E H I D R K F V P F R K L Q F G R Y P G A F D R P E L Q Q V T V D G L E V L I P K D P M H F V E E V P H S R F I E C R Y K E A R A F F Q Q Y L D D N T V E A V A F R K S A K E L L Q L A A K T L N K L G V P F W L S S G T C L G W Y R Q C N I I P Y S K D V D L G I F I Q D Y K S D I I L A F Q D A G L P L K H K F G K V E D S L E L S F Q G K D D V K L D V F F F Y E E T D H M W N G G T Q A K T G K K F K Y L F P K F T L C W T E F V D M K V H V P C E T L E Y I E A N Y G K T W K I P V K T W D W K R S P P N V Q P N G I W P I S E W D E V I Q L Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:26923585, PubMed:27194101, PubMed:29477842). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (PubMed:17034757, PubMed:25279699, PubMed:26923585, PubMed:27194101, PubMed:29477842). Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity (PubMed:17034757). May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes (PubMed:25279699). Could be involved in brain development (Probable).
Alternative names
FCMD, FKTN, Ribitol-5-phosphate transferase FKTN, Fukutin, Fukuyama-type congenital muscular dystrophy protein, Ribitol-5-phosphate transferase
Recommended products
Recombinant Human FKTN Protein (His Tag) is a Human Full Length protein, in the 1 to 461 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- 10x His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75072-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol
Sequence info
Amino acid sequence
- M S R I N K N V V L A L L T L T S S A F L L F Q L Y Y Y K H Y L S T K N G A G L S K S K G S R I G F D S T Q W R A V K K F I M L T S N Q N V P V F L I D P L I L E L I N K N F E Q V K N T S H G S T S Q C K F F C V P R D F T A F A L Q Y H L W K N E E G W F R I A E N M G F Q C L K I E S K D P R L D G I D S L S G T E I P L H Y I C K L A T H A I H L V V F H E R S G N Y L W H G H L R L K E H I D R K F V P F R K L Q F G R Y P G A F D R P E L Q Q V T V D G L E V L I P K D P M H F V E E V P H S R F I E C R Y K E A R A F F Q Q Y L D D N T V E A V A F R K S A K E L L Q L A A K T L N K L G V P F W L S S G T C L G W Y R Q C N I I P Y S K D V D L G I F I Q D Y K S D I I L A F Q D A G L P L K H K F G K V E D S L E L S F Q G K D D V K L D V F F F Y E E T D H M W N G G T Q A K T G K K F K Y L F P K F T L C W T E F V D M K V H V P C E T L E Y I E A N Y G K T W K I P V K T W D W K R S P P N V Q P N G I W P I S E W D E V I Q L Y
- Accession
- O75072
- Protein length
- Full Length
- Actual molecular weight
- 56.5 kDa
- Amino acids
- 1 to 461
- Nature
- Recombinant
- Tags
- 10x His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:26923585, PubMed:27194101, PubMed:29477842). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (PubMed:17034757, PubMed:25279699, PubMed:26923585, PubMed:27194101, PubMed:29477842). Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity (PubMed:17034757). May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes (PubMed:25279699). Could be involved in brain development (Probable).
- Sequence similarities
Belongs to the LicD transferase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fukutin also known as FKRP or FKTN is a glycosyltransferase enzyme weighing approximately 55 kDa. This protein participates in the process of adding sugar chains to proteins primarily glycosylating α-dystroglycan. Fukutin is highly expressed in muscle tissues including skeletal and cardiac muscles and plays a role in the central nervous system. Its activity is important for the proper function of these tissues.
- Biological function summary
Fukutin plays a fundamental role in maintaining the structure and function of muscle cells by participating in the glycosylation of α-dystroglycan. This modification allows α-dystroglycan to bind to extracellular matrix proteins. Fukutin operates as part of the dystrophin-glycoprotein complex which is essential for muscle integrity and stability. This complex anchors the cytoskeleton of a muscle cell to the surrounding extracellular matrix.
- Pathways
This protein fukutin participates in the dystroglycan pathway affecting the integrity of muscle cells. It works closely with other proteins like LARGE1 which also modifies α-dystroglycan. Fukutin's activity ensures that α-dystroglycan has the necessary post-translational modifications to perform its function within the laminin-binding pathway linking the extracellular matrix to the cytoskeleton.
- Associated diseases and disorders
Alterations in fukutin mutations can lead to muscular dystrophies such as Fukuyama congenital muscular dystrophy and Walker-Warburg syndrome. In these conditions the fukutin-related glycosylation of α-dystroglycan is disrupted which impairs muscle cell stability and function. Other proteins like dystrophin and sarcoglycans associated with the dystrophin-glycoprotein complex also play roles in these disorders further linking fukutin to muscle disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Human FKTN Protein (His Tag) (ab314618)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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