Recombinant Human FLNA protein is a Human Full Length protein, in the 1 to 838 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P S G K V A Q P T I T D N K D G T V T V R Y A P S E A G L H E M D I R Y D N M H I P G S P L Q F Y V D Y V N C G H V T A Y G P G L T H G V V N K P A T F T V N T K D A G E G G L S L A I E G P S K A E I S C T D N Q D G T C S V S Y L P V L P G D Y S I L V K Y N E Q H V P G S P F T A R V T G D D S M R M S H L K V G S A A D I P I N I S E T D L S L L T A T V V P P S G R E E P C L L K R L R N G H V G I S F V P K E T G E H L V H V K K N G Q H V A S S P I P V V I S Q S E I G D A S R V R V S G Q G L H E G H T F E P A E F I I D T R D A G Y G G L S L S I E G P S K V D I N T E D L E D G T C R V T Y C P T E P G N Y I I N I K F A D Q H V P G S P F S V K V T G E G R V K E S I T R R R R A P S V A N V G S H C D L S L K I P E I S I Q D M T A Q V T S P S G K T H E A E I V E G E N H T Y C I R F V P A E M G T H T V S V K Y K G Q H V P G S P F Q F T V G P L G E G G A H K V R A G G P G L E R A E A G V P A E F S I W T R E A G A G G L A I A V E G P S K A E I S F E D R K D G S C G V A Y V V Q E P G D Y E V S V K F N E E H I P D S P F V V P V A S P S G D A R R L T V S S L Q E S G L K V N Q P A S F A V S L N G A K G A I D A K V H S P S G A L E E C Y V T E I D Q D K Y A V R F I P R E N G V Y L I D V K F N G T H I P G S P F K I R V G E P G H G G D P G L V S A Y G A G L E G G V T G N P A E F V V N T S N A G A G A L S V T I D G P S K V K M D C Q E C P E G Y R V T Y T P M A P G S Y L I S I K Y G G P Y H I G G S P F K A K V T G P R L V S N H S L H E T S S V F V D S L T K A T C A P Q H G A P G P G P A D A S K V V A K G L G L S K A Y V G Q K S S F T V D C S K A G N N M L L V G V H G P R T P C E E I L V K H V G S R L Y S V S Y L L K D K G E Y T L V V K W G D E H I P G S P Y R V V V P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity). During the axon guidance process, required for growth cone collapse induced by SEMA3A-mediated stimulation of neurons (PubMed:25358863).
Alternative names
FLN, FLN1, FLNA, Filamin-A, FLN-A, Actin-binding protein 280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, ABP-280
Recommended products
Recombinant Human FLNA protein is a Human Full Length protein, in the 1 to 838 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P21333-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P S G K V A Q P T I T D N K D G T V T V R Y A P S E A G L H E M D I R Y D N M H I P G S P L Q F Y V D Y V N C G H V T A Y G P G L T H G V V N K P A T F T V N T K D A G E G G L S L A I E G P S K A E I S C T D N Q D G T C S V S Y L P V L P G D Y S I L V K Y N E Q H V P G S P F T A R V T G D D S M R M S H L K V G S A A D I P I N I S E T D L S L L T A T V V P P S G R E E P C L L K R L R N G H V G I S F V P K E T G E H L V H V K K N G Q H V A S S P I P V V I S Q S E I G D A S R V R V S G Q G L H E G H T F E P A E F I I D T R D A G Y G G L S L S I E G P S K V D I N T E D L E D G T C R V T Y C P T E P G N Y I I N I K F A D Q H V P G S P F S V K V T G E G R V K E S I T R R R R A P S V A N V G S H C D L S L K I P E I S I Q D M T A Q V T S P S G K T H E A E I V E G E N H T Y C I R F V P A E M G T H T V S V K Y K G Q H V P G S P F Q F T V G P L G E G G A H K V R A G G P G L E R A E A G V P A E F S I W T R E A G A G G L A I A V E G P S K A E I S F E D R K D G S C G V A Y V V Q E P G D Y E V S V K F N E E H I P D S P F V V P V A S P S G D A R R L T V S S L Q E S G L K V N Q P A S F A V S L N G A K G A I D A K V H S P S G A L E E C Y V T E I D Q D K Y A V R F I P R E N G V Y L I D V K F N G T H I P G S P F K I R V G E P G H G G D P G L V S A Y G A G L E G G V T G N P A E F V V N T S N A G A G A L S V T I D G P S K V K M D C Q E C P E G Y R V T Y T P M A P G S Y L I S I K Y G G P Y H I G G S P F K A K V T G P R L V S N H S L H E T S S V F V D S L T K A T C A P Q H G A P G P G P A D A S K V V A K G L G L S K A Y V G Q K S S F T V D C S K A G N N M L L V G V H G P R T P C E E I L V K H V G S R L Y S V S Y L L K D K G E Y T L V V K W G D E H I P G S P Y R V V V P
- Protein length
- Full Length
- Predicted molecular weight
- 118 kDa
- Amino acids
- 1 to 838
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity). During the axon guidance process, required for growth cone collapse induced by SEMA3A-mediated stimulation of neurons (PubMed:25358863).
- Sequence similarities
Belongs to the filamin family.
- Post-translational modifications
Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human FLNA protein (ab131760)
12.5% SDS-PAGE stained with Coomassie Blue showing ab131760.
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Product protocols
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