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AB79183

Recombinant Human FLR protein (Tag Free)

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Recombinant Human FLR protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

FLR, SCAN, BLVRB, Flavin reductase (NADPH), FR, Biliverdin reductase B, Biliverdin-IX beta-reductase, Green heme-binding protein, NADPH-dependent diaphorase, NADPH-flavin reductase, S-nitroso-CoA-assisted nitrosyltransferase, BVR-B, GHBP, SNO-CoA-assisted nitrosyltransferase

1 Images
SDS-PAGE - Recombinant Human FLR protein (Tag Free) (AB79183)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FLR protein (Tag Free) (AB79183)

15% SDS-PAGE showing ab79183 at approximately 22kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P30043

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Recombinant Human eIF5A protein (Tag Free)

Western blot - Recombinant Human eIF5A protein (Tag Free) (AB87457)

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Primary Antibodies

AB276064

Anti-FLR antibody [009] - BSA and Azide free

Recombinant

Immunoprecipitation - Anti-FLR antibody [009] - BSA and Azide free (AB276064)

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Proteins & Peptides

AB140552

Recombinant Human RDHE2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human RDHE2 protein (His tag N-Terminus) (AB140552)

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Proteins & Peptides

AB104668

Recombinant Human hnRNP C1/C2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human hnRNP C1/C2 protein (His tag N-Terminus) (AB104668)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as BLVRB
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Sequence info

[{"sequence":"MAVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30043","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab79183 is purified using conventional chromatography techniques.

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General info

Function

Enzyme that can both act as a NAD(P)H-dependent reductase and a S-nitroso-CoA-dependent nitrosyltransferase (PubMed : 10620517, PubMed : 18241201, PubMed : 27207795, PubMed : 38056462, PubMed : 7929092). Promotes fetal heme degradation during development (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Also expressed in adult tissues, where it acts as a regulator of hematopoiesis, intermediary metabolism (glutaminolysis, glycolysis, TCA cycle and pentose phosphate pathway) and insulin signaling (PubMed : 27207795, PubMed : 29500232, PubMed : 38056462). Has a broad specificity oxidoreductase activity by catalyzing the NAD(P)H-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone) (PubMed : 10620517, PubMed : 18241201, PubMed : 7929092). Contributes to fetal heme catabolism by catalyzing reduction of biliverdin IXbeta into bilirubin IXbeta in the liver (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Biliverdin IXbeta, which constitutes the major heme catabolite in the fetus is not present in adult (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Does not reduce bilirubin IXalpha (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH (PubMed : 10620517). Acts as a protein nitrosyltransferase by catalyzing nitrosylation of cysteine residues of target proteins, such as HMOX2, INSR and IRS1 (PubMed : 38056462). S-nitroso-CoA-dependent nitrosyltransferase activity is mediated via a 'ping-pong' mechanism : BLVRB first associates with both S-nitroso-CoA and protein substrate, nitric oxide group is then transferred from S-nitroso-CoA to Cys-109 and Cys-188 residues of BLVRB and from S-nitroso-BLVRB to the protein substrate (PubMed : 38056462). Inhibits insulin signaling by mediating nitrosylation of INSR and IRS1, leading to their inhibition (PubMed : 38056462).

Sequence similarities

Belongs to the BLVRB family.

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Product protocols

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Target data

Enzyme that can both act as a NAD(P)H-dependent reductase and a S-nitroso-CoA-dependent nitrosyltransferase (PubMed : 10620517, PubMed : 18241201, PubMed : 27207795, PubMed : 38056462, PubMed : 7929092). Promotes fetal heme degradation during development (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Also expressed in adult tissues, where it acts as a regulator of hematopoiesis, intermediary metabolism (glutaminolysis, glycolysis, TCA cycle and pentose phosphate pathway) and insulin signaling (PubMed : 27207795, PubMed : 29500232, PubMed : 38056462). Has a broad specificity oxidoreductase activity by catalyzing the NAD(P)H-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone) (PubMed : 10620517, PubMed : 18241201, PubMed : 7929092). Contributes to fetal heme catabolism by catalyzing reduction of biliverdin IXbeta into bilirubin IXbeta in the liver (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Biliverdin IXbeta, which constitutes the major heme catabolite in the fetus is not present in adult (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Does not reduce bilirubin IXalpha (PubMed : 10858451, PubMed : 18241201, PubMed : 7929092). Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH (PubMed : 10620517). Acts as a protein nitrosyltransferase by catalyzing nitrosylation of cysteine residues of target proteins, such as HMOX2, INSR and IRS1 (PubMed : 38056462). S-nitroso-CoA-dependent nitrosyltransferase activity is mediated via a 'ping-pong' mechanism : BLVRB first associates with both S-nitroso-CoA and protein substrate, nitric oxide group is then transferred from S-nitroso-CoA to Cys-109 and Cys-188 residues of BLVRB and from S-nitroso-BLVRB to the protein substrate (PubMed : 38056462). Inhibits insulin signaling by mediating nitrosylation of INSR and IRS1, leading to their inhibition (PubMed : 38056462).
See full target information BLVRB
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