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Recombinant human Flt3 / CD135 protein is a Human Fragment protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for Inhib.
Alternative names=Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, FLK-2, FLT-3, STK-1, CD135, FLK2, STK1, FLT3
- Purity
>80% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag N-Terminus
- Applications
Inhib
- Biologically active
Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Inhib | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Recombinant human Flt3 / CD135 protein is a Human Fragment protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for Inhib.
Alternative names=Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, FLK-2, FLT-3, STK-1, CD135, FLK2, STK1, FLT3
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag N-Terminus
- Applications
Inhib
- Biologically active
Yes
- Biological activity
Activity: 20 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30 C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant Flt3.
- Predicted molecular weight
54.2 kDa
- Accession
- P36888-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Tween, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Post-translational modifications
N-glycosylated, contains complex N-glycans with sialic acid.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein was expressed in a Baculovirus infected Sf9 cell expression system. Expected molecular weight of 54.2 kDa.
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