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AB223021

Recombinant Human Folate Binding Protein/FBP (His tag)

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Recombinant Human Folate Binding Protein/FBP (His tag) is a Human Fragment protein, in the 26 to 234 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

FOLR, FOLR1, Folate receptor alpha, FR-alpha, Adult folate-binding protein, Folate receptor 1, KB cells FBP, Ovarian tumor-associated antigen MOv18, FBP

1 Images
SDS-PAGE - Recombinant Human Folate Binding Protein/FBP (His tag) (AB223021)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Folate Binding Protein/FBP (His tag) (AB223021)

3 μg of ab223021 analyzed by 15% SDS-PAGE.

28-40kDa (under reducing conditions).

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P15328

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Folate Binding Protein
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Sequence info

[{"sequence":"ADLIAWARTELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHFIQDTCLYECSPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWTSGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAAAMSHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"25.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":234,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P15328","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Folate Binding Protein (FBP) also known as FolR1 protein or FOLR-1 is a significant protein with a mass of about 32-38 kDa. It binds folate with high affinity and facilitates its transport into the cell. FBP can be found in various tissues with notable expression in the placenta kidney and certain epithelial tissues. The protein mediates folate uptake by capturing folates in the extracellular matrix and channeling them through receptor-mediated endocytosis.
Biological function summary

FBP plays a critical role in cellular growth and replication by ensuring sufficient folate supply. It belongs to a family of receptors not forming part of a larger protein complex. FBP's primary function is delivering folate an important B vitamin which acts as a one-carbon donor in DNA synthesis and repair. Optimal folate transport by FBP supports cellular functions particularly in rapidly dividing cells.

Pathways

FBP significantly contributes to the folate-mediated one-carbon metabolic pathway critical for nucleotide biosynthesis. This pathway interconnects with the homocysteine remethylation cycle. FBP associates with other enzymes such as dihydrofolate reductase (DHFR) and methylenetetrahydrofolate reductase (MTHFR) which also play key roles in these pathways. These interactions ensure efficient folate metabolism and maintenance of normal cellular functions.

FBP's relation to cancer and neural tube defects is well documented. Overexpression of FBP has been observed in various cancers including ovarian and breast cancer which indicates its potential role in tumor progression. In neural tube defects altered folate transport due to variations in FBP expression impacts neural development. FBP connects to other markers like HER2 in cancer which may form part of a therapeutic target strategy.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed : 19074442, PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed : 8567728). Required for normal embryonic development and normal cell proliferation (By similarity).

Sequence similarities

Belongs to the folate receptor family.

Post-translational modifications

The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.

Subcellular localisation

Endosome

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Product protocols

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Target data

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed : 19074442, PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed : 8567728). Required for normal embryonic development and normal cell proliferation (By similarity).
See full target information FOLR1
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