Recombinant Human Fos B protein is a Human Fragment protein, in the 50 to 338 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P G S F V P T V T A I T T S Q D L Q W L V Q P T L I S S M A Q S Q G Q P L A S Q P P V V D P Y D M P G T S Y S T P G M S G Y S S G G A S G S G G P S T S G T T S G P G P A R P A R A R P R R P R E E T L T P E E E E K R R V R R E R N K L A A A K C R N R R R E L T D R L Q A E T D Q L E E E K A E L E S E I A E L Q K E K E R L E F V L V A H K P G C K I P Y E E G P G P G P L A E V R D L P G S A P A K E D G F S W L L P P P P P P P L P F Q T S Q D A P P N L T A S L F T H S E V Q V L G D P F P V V N P S Y T S S F V L T C P E V S A F A G A Q R T S G S D Q P S D P L N S P S L L A L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Heterodimerizes with proteins of the JUN family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to gene promoters containing an AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing their transcriptional activity (PubMed:12618758, PubMed:28981703). As part of the AP-1 complex, facilitates enhancer selection together with cell-type-specific transcription factors by collaboratively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (By similarity). Together with JUN, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Exhibits transactivation activity in vitro (By similarity). Involved in the display of nurturing behavior towards newborns (By similarity). May play a role in neurogenesis in the hippocampus and in learning and memory-related tasks by regulating the expression of various genes involved in neurogenesis, depression and epilepsy (By similarity). Implicated in behavioral responses related to morphine reward and spatial memory (By similarity). Isoform 11. Exhibits lower transactivation activity than isoform 1 in vitro (By similarity). The heterodimer with JUN does not display any transcriptional activity, and may thereby act as an transcriptional inhibitor (By similarity). May be involved in the regulation of neurogenesis in the hippocampus (By similarity). May play a role in synaptic modifications in nucleus accumbens medium spiny neurons and thereby play a role in adaptive and pathological reward-dependent learning, including maladaptive responses involved in drug addiction (By similarity). Seems to be more stably expressed with a half-life of ~9.5 hours in cell culture as compared to 1.5 hours half-life of isoform 1 (By similarity).
Alternative names
G0S3, FOSB, Protein FosB, G0/G1 switch regulatory protein 3, Transcription factor AP-1 subunit FosB
Recommended products
Recombinant Human Fos B protein is a Human Fragment protein, in the 50 to 338 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P53539-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P G S F V P T V T A I T T S Q D L Q W L V Q P T L I S S M A Q S Q G Q P L A S Q P P V V D P Y D M P G T S Y S T P G M S G Y S S G G A S G S G G P S T S G T T S G P G P A R P A R A R P R R P R E E T L T P E E E E K R R V R R E R N K L A A A K C R N R R R E L T D R L Q A E T D Q L E E E K A E L E S E I A E L Q K E K E R L E F V L V A H K P G C K I P Y E E G P G P G P L A E V R D L P G S A P A K E D G F S W L L P P P P P P P L P F Q T S Q D A P P N L T A S L F T H S E V Q V L G D P F P V V N P S Y T S S F V L T C P E V S A F A G A Q R T S G S D Q P S D P L N S P S L L A L
- Accession
- P53539
- Protein length
- Fragment
- Predicted molecular weight
- 57.4 kDa
- Amino acids
- 50 to 338
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Heterodimerizes with proteins of the JUN family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to gene promoters containing an AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing their transcriptional activity (PubMed:12618758, PubMed:28981703). As part of the AP-1 complex, facilitates enhancer selection together with cell-type-specific transcription factors by collaboratively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (By similarity). Together with JUN, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Exhibits transactivation activity in vitro (By similarity). Involved in the display of nurturing behavior towards newborns (By similarity). May play a role in neurogenesis in the hippocampus and in learning and memory-related tasks by regulating the expression of various genes involved in neurogenesis, depression and epilepsy (By similarity). Implicated in behavioral responses related to morphine reward and spatial memory (By similarity).
- Sequence similarities
Belongs to the bZIP family. Fos subfamily.
- Post-translational modifications
Phosphorylated.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fos B also known as FosB is a protein encoded by the FOSB gene and belongs to the Fos family of transcription factors. Weighing approximately 46 kDa this protein acts within the cell nucleus and is expressed in several tissues particularly in brain regions. Delta Fos B an isoform of Fos B has gained attention for its stable accumulation in certain neuronal populations. Researchers often study Fos B using techniques like ELISA and immunohistochemistry to investigate its presence and distribution.
- Biological function summary
Functions of Fos B include regulation of gene expression through forming AP-1 transcription factor complexes with Jun family proteins. These complexes bind to DNA influencing the expression of genes involved in processes such as cell proliferation differentiation and survival. Fos B is structurally similar to other Fos family proteins such as c-Fos which emphasises its role in response to extracellular signals and stress stimuli. Both Fos B and its isoform Delta Fos B have distinct expression patterns suggesting unique functions.
- Pathways
Fos B plays a role in the MAPK/ERK signaling pathway which is essential for transmitting extracellular signals to promote diverse cellular responses. Within this pathway interactions with proteins like Jun lead to modulation of gene expression critical for cellular responses to growth factors. Additionally connections to the CREB signaling pathway link Fos B to processes concerning neuronal plasticity and memory further highlighting its participation in important biological functions.
- Associated diseases and disorders
Involvement of Fos B has been noted in neurological conditions such as addiction and depression. Delta Fos B in particular accumulates in response to chronic drug exposure and serves as a molecular switch in addiction-related neuroadaptations. Connections with proteins such as CREB and other transcriptional regulators have broadened the understanding of how Fos B and its isoforms impact neural circuits and contribute to the pathology of mood disorders.
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1 product image
SDS-PAGE - Recombinant Human Fos B protein (ab152396)
12.5% SDS-PAGE analysis of ab152396 stained with Coomassie Blue.
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