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AB134839

Recombinant Human FOXA3 protein

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Recombinant Human FOXA3 protein is a Human Full Length protein, in the 2 to 350 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, CellDiff.

View Alternative Names

HNF3G, TCF3G, FOXA3, Hepatocyte nuclear factor 3-gamma, HNF-3-gamma, HNF-3G, Fork head-related protein FKH H3, Forkhead box protein A3, Transcription factor 3G, TCF-3G

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, CellDiff

applications

Biologically active

No

Accession

P55318

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "CellDiff": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2-8 µg/mL", "notes":"<p>ab134839 may be used for studying of Human liver cell differentiation in vitro.</p>" } } }
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Sequence info

[{"sequence":"29 amino acid tag-GEFLGSVKMEAHDLAEWSYYPEAGEVYSPVTPVPTMAPLNSYMTLNPLSSPYPPGGLPASPLPSGPLAPPAPAAPLGPTFPGLGVSGGSSSSGYGAPGPGLVHGKEMPKGYRRPLAHAKPPYSYISLITMAIQQAPGKMLTLSEIYQWIMDLFPYYRENQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSYWALHPSSGNMFENGCYLRRQKRFKLEEKVKKGGSGAATTTRNGTGSAASTTTPAATVTSPPQPPPPAPEPEAQGGEDVGALDCGSPASSTPYFTGLELPGELKLDAPYNFNHPFSINNLMSEQTPAPPKLDVGFGGYGAEGGEPGVYYQGLYSRSLLNASLEESGGGGSPGRRRRRRRRRRR","proteinLength":"Full Length","predictedMolecularWeight":"37 kDa","actualMolecularWeight":null,"aminoAcidEnd":350,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P55318","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXA3 also known as Forkhead box protein A3 is a transcription factor that belongs to the FOX family. It has a molecular mass of approximately 43 kDa. FOXA3 exhibits expression mainly in the liver pancreas and lung tissues where it regulates the transcription of genes involved in metabolic processes. It has a highly conserved DNA-binding domain known as the forkhead or winged helix domain which allows binding to specific DNA sequences to control gene expression.
Biological function summary

FOXA3 plays an important role in the regulation of glucose and lipid homeostasis. It promotes the expression of genes involved in gluconeogenesis and lipogenesis processes. As a component of a transcriptional complex FOXA3 interacts with other transcription factors and co-activators to modulate target gene transcription. Its regulatory activities help maintain the balance of energy supply and storage in response to physiological needs.

Pathways

FOXA3 participates in significant metabolic pathways that are essential for energy production and storage. One important pathway involves gluconeogenesis where FOXA3 partners with other transcription factors such as HNF4α to promote the expression of genes required for glucose synthesis. Additionally FOXA3 is involved in lipid metabolism pathways coordinating with proteins like PPARG to regulate lipogenic genes ensuring proper lipid storage and utilization in cells.

FOXA3 is linked to conditions like type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Aberrant regulation of FOXA3 can disturb glucose and lipid metabolism contributing to the development of these metabolic disorders. FOXA3 interacts with proteins such as PPARG and HNF4α which also have implications in these diseases by modulating pathways that affect insulin sensitivity and fat accumulation. Proper function of FOXA3 is essential for maintaining metabolic health.
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Specifications

Form

Liquid

Additional notes

ab134839 was expressed in E. coli as inclusion bodies, refolded using a temperature shift inclusion body refolding technology and chromatographically purified.

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General info

Function

Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC1 promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC1 promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.
See full target information FOXA3
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