Recombinant Human FRZB protein (denatured) is a Human Full Length protein, in the 33 to 325 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S A A C E P V R I P L C K S L P W N M T K M P N H L H H S T Q A N A I L A I E Q F E G L L G T H C S P D L L F F L C A M Y A P I C T I D F Q H E P I K P C K S V C E R A R Q G C E P I L I K Y R H S W P E N L A C E E L P V Y D R G V C I S P E A I V T A D G A D F P M D S S N G N C R G A S S E R C K C K P I R A T Q K T Y F R N N Y N Y V I R A K V K E I K T K C H D V T A V V E V K E I L K S S L V N I P R D T V N L Y T S S G C L C P P L N V N E E Y I I M G Y E D E E R S R L L L V E G S I A E K W K D R L G K K V K R W D M K L R H L G L S K S D S S N S D S T Q S Q K S G R N S N P R Q A R N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
Alternative names
FIZ, FRE, FRP, FRZB1, SFRP3, FRZB, Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1
Recommended products
Recombinant Human FRZB protein (denatured) is a Human Full Length protein, in the 33 to 325 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q92765-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S A A C E P V R I P L C K S L P W N M T K M P N H L H H S T Q A N A I L A I E Q F E G L L G T H C S P D L L F F L C A M Y A P I C T I D F Q H E P I K P C K S V C E R A R Q G C E P I L I K Y R H S W P E N L A C E E L P V Y D R G V C I S P E A I V T A D G A D F P M D S S N G N C R G A S S E R C K C K P I R A T Q K T Y F R N N Y N Y V I R A K V K E I K T K C H D V T A V V E V K E I L K S S L V N I P R D T V N L Y T S S G C L C P P L N V N E E Y I I M G Y E D E E R S R L L L V E G S I A E K W K D R L G K K V K R W D M K L R H L G L S K S D S S N S D S T Q S Q K S G R N S N P R Q A R N
- Accession
- Q92765
- Protein length
- Full Length
- Predicted molecular weight
- 35 kDa
- Amino acids
- 33 to 325
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
- Sequence similarities
Belongs to the secreted frizzled-related protein (sFRP) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FRZB also known as secreted frizzled-related protein 3 (SFRP3) functions as a modulator of Wnt signaling pathways. This protein has a mass of approximately 36 kDa and serves as an antagonist to the Wnt proteins by binding to them preventing their interaction with cell surface receptors. FRZB is expressed in several tissues with notable levels in cartilage bone and the heart.
- Biological function summary
The modulation of Wnt signaling by FRZB plays a significant role in embryonic development and tissue homeostasis. FRZB is not part of a protein complex but has important interactions with other components of the signaling cascade. It influences cell differentiation proliferation and apoptosis which are critical processes during development and tissue repair.
- Pathways
FRZB influences both Wnt/β-catenin and non-canonical Wnt pathways. Its interaction with these pathways regulates signal transmission that affects cellular behaviors. Related proteins include Wnt ligands and frizzled receptors which are central to these signaling mechanisms.
- Associated diseases and disorders
Abnormalities in FRZB expression or function show a connection to osteoarthritis and certain cancers. In osteoarthritis FRZB interacts with Wnt proteins which contribute to the degradation of cartilage. In cancer dysregulation of Wnt signaling involving FRZB can lead to unchecked cell proliferation. Understanding its role in these diseases can aid in developing therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human FRZB protein (denatured) (ab139224)
15% SDS-PAGE analysis of 3 µg ab139224.
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