Recombinant Human FSBP protein (denatured) is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M V G K A R S S N F T L S E K L D L L K L V K P Y V K I L E E H T N K H S V I V E K N R C W D I I A V N Y N A I G V D R P P R T A Q G L R T L Y K R L K E Y A K Q E L L Q Q K E T Q S D F K S N I S E P T K K V M E M I P Q I S S F C L V R D R N H I Q S A N L D E E A Q A G T S S L Q V M L D H H P V A I T V E V K Q E E D I K P P P P L V L N S Q Q S D T L E Q R E E H E L V H V M E R S L S P S L S S V D M R M T S S P S S I P R R D D F F R H E S G E H F R S L L G Y D P Q I L Q M L K E E H Q I I L E N Q K N F G L Y V Q E K R D G L K R R Q Q L E E E L L R A K I E V E K L K A I R L R H D L P E Y N S L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional repressor that down-regulates the expression of the fibrinogen gamma chain. Represses transcription of GSK3B gene promoter via its interaction with APBA1.
Alternative names
Fibrinogen silencer-binding protein, FSBP
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Recombinant Human FSBP protein (denatured) is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O95073-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 6% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M V G K A R S S N F T L S E K L D L L K L V K P Y V K I L E E H T N K H S V I V E K N R C W D I I A V N Y N A I G V D R P P R T A Q G L R T L Y K R L K E Y A K Q E L L Q Q K E T Q S D F K S N I S E P T K K V M E M I P Q I S S F C L V R D R N H I Q S A N L D E E A Q A G T S S L Q V M L D H H P V A I T V E V K Q E E D I K P P P P L V L N S Q Q S D T L E Q R E E H E L V H V M E R S L S P S L S S V D M R M T S S P S S I P R R D D F F R H E S G E H F R S L L G Y D P Q I L Q M L K E E H Q I I L E N Q K N F G L Y V Q E K R D G L K R R Q Q L E E E L L R A K I E V E K L K A I R L R H D L P E Y N S L
- Accession
- O95073
- Protein length
- Full Length
- Predicted molecular weight
- 37.2 kDa
- Amino acids
- 1 to 299
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcriptional repressor that down-regulates the expression of the fibrinogen gamma chain. Represses transcription of GSK3B gene promoter via its interaction with APBA1.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FSBP also known as Fructose-1-Six-Bisphosphatase acts as an important enzyme in the hydrolysis of fructose-16-bisphosphate to fructose 6-phosphate in the gluconeogenesis pathway. Its molecular mass is approximately 37 kDa. FSBP expresses mainly in the liver kidney and muscle tissues where it plays a critical role in glucose metabolism. The enzyme exists in two isoforms: FBP1 and FBP2 with FBP1 expressed mainly in the liver while FBP2 appears more in skeletal muscle.
- Biological function summary
FSBP regulates blood sugar levels by facilitating gluconeogenesis the process of producing glucose from non-carbohydrate sources. FSBP functions as part of a larger complex that includes PFK/FBPase which maintains the balance between glycolysis and gluconeogenesis. The presence of FSBP ensures that cells receive adequate glucose supply which is essential for energy production particularly during fasting conditions.
- Pathways
FSBP occupies an important position in the gluconeogenesis and glycolysis metabolic pathways. It directly opposes the action of phosphofructokinase-1 (PFK-1) a rate-limiting enzyme in glycolysis. The regulation of these pathways relies on reciprocal control where FSBP decreases glycolytic flow to promote gluconeogenesis and maintain blood glucose homeostasis. By interacting with PFK-1 FSBP ensures a seamless transition between energy storage and energy release in cells.
- Associated diseases and disorders
FSBP plays a significant role in conditions such as fructose-16-bisphosphatase deficiency and diabetes mellitus. Deficiency in FSBP can lead to an inability to perform gluconeogenesis effectively causing hypoglycemia and lactic acidosis. In diabetes mellitus alteration in FSBP activity affects glucose homeostasis and may contribute to hyperglycemia. The enzyme's interaction with glucose-6-phosphatase in gluconeogenesis further highlights its importance in these metabolic disorders.
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1 product image
SDS-PAGE - Recombinant Human FSBP protein (denatured) (ab156713)
15% SDS-PAGE analysis of ab156713 (3μg).
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