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AB131684

Recombinant Human FTSJ2 protein (His tag N-Terminus)

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Recombinant Human FTSJ2 protein (His tag N-Terminus) is a Human Fragment protein, in the 51 to 246 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

FJH1, FTSJ2, MRM2, 16S rRNA (uridine(1369)-2'-O)-methyltransferase, 16S rRNA [Um1369] 2'-O-methyltransferase, Protein ftsJ homolog 2

1 Images
SDS-PAGE - Recombinant Human FTSJ2 protein (His tag N-Terminus) (AB131684)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FTSJ2 protein (His tag N-Terminus) (AB131684)

15% SDS-PAGE analysis of 3 µg ab131684.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9UI43

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSYRCRSAFKLLEVNERHQILRPGLRVLDCGAAPGAWSQVAVQKVNAAGTDPSSPVGFVLGVDLLHIFPLEGATFLCPADVTDPRTSQRILEVLPGRRADVILSDMAPNATGFRDLDHDRLISLCLTLLSVTPDILQPGGTFLCKTWAGSQSRRLQRRLTEEFQNVRIIKPEASRKESSEVYFLATQYHGRKGTVKQ","proteinLength":"Fragment","predictedMolecularWeight":"24.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":51,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UI43","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FTSJ2 also known as hPrmA or methyltransferase-like protein 1 is an enzyme with a mass of approximately 83 kDa. It functions as a human 2'-O-methyltransferase and takes part in ribosomal RNA modification by methylating the ribose moiety in RNA molecules. You can find FTSJ2 widely expressed in various human tissues with notable expression in the liver and brain. The enzyme localizes mainly in the nucleolus a site central to ribosomal RNA biosynthesis and modification activities.
Biological function summary

The enzymatic activities of FTSJ2 contribute to ribosome assembly and stability by modifying specific rRNA components. These modifications are essential for the accurate assembly of ribosomal subunits and the proper function of the translation machinery within the cell. FTSJ2 does not function in isolation; it often operates as part of multiprotein complexes which assist in guiding and regulating its methyltransferase activity.

Pathways

Methylation activity by FTSJ2 plays a critical role in the RNA processing pathway and cellular translation machinery. It directly affects the biogenesis of rRNA ensuring the efficient assembly of ribosomal units required for protein synthesis. FTSJ2 interacts with other proteins such as NOP10 and NOP56 which are potentials linking it to the broader ribonucleoprotein complex within cells.

Alterations in FTSJ2 activity and expression have connections to several human conditions notably cancer and neurological disorders. Aberrations in the rRNA modification process could lead to disruptions in protein synthesis and uncontrolled cell proliferation which are often observed in cancerous cells. In the context of neurological disorders FTSJ2's function links to proteins like FBL which play key roles in neural cell RNA processing. Changes in their function could potentially contribute to neurodegenerative conditions.
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Specifications

Form

Liquid

Additional notes

ab131684 was purified using conventional chromatography techniques.

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General info

Function

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA (PubMed : 25009282, PubMed : 25074936, PubMed : 35177605). This activity may require prior 2'-O-methylguanosine modification at position 1370 (Gm1370) by MRM3 (PubMed : 35177605). Essential for late-stage assembly of mtLSU required for efficient translation of mitochondrial DNA encoded proteins; methyltransferase activity is not required for this function (PubMed : 35177605). Essential for mitochondrial respiratory function (PubMed : 35177605).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA (PubMed : 25009282, PubMed : 25074936, PubMed : 35177605). This activity may require prior 2'-O-methylguanosine modification at position 1370 (Gm1370) by MRM3 (PubMed : 35177605). Essential for late-stage assembly of mtLSU required for efficient translation of mitochondrial DNA encoded proteins; methyltransferase activity is not required for this function (PubMed : 35177605). Essential for mitochondrial respiratory function (PubMed : 35177605).
See full target information MRM2
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Complementary Products

Primary Antibodies

AB60068

Anti-FTSJ2 antibody [2372C6a]

Western blot - Anti-FTSJ2 antibody [2372C6a] (AB60068)

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