Recombinant Human FUBP1/FBP protein is a Human Full Length protein, in the 1 to 653 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A D Y S T V P P P S S G S A G G G G G G G G G G G V N D A F K D A L Q R A R Q I A A K I G G D A G T S L N S N D Y G Y G G Q K R P L E D G D Q P D A K K V A P Q N D S F G T Q L P P M H Q Q Q R S V M T E E Y K V P D G M V G F I I G R G G E Q I S R I Q Q E S G C K I Q I A P D S G G L P E R S C M L T G T P E S V Q S A K R L L D Q I V E K G R P A P G F H H G D G P G N A V Q E I M I P A S K A G L V I G K G G E T I K Q L Q E R A G V K M V M I Q D G P Q N T G A D K P L R I T G D P Y K V Q Q A K E M V L E L I R D Q G G F R E V R N E Y G S R I G G N E G I D V P I P R F A V G I V I G R N G E M I K K I Q N D A G V R I Q F K P D D G T T P E R I A Q I T G P P D R C Q H A A E I I T D L L R S V Q A G N P G G P G P G G R G R G R G Q G N W N M G P P G G L Q E F N F I V P T G K T G L I I G K G G E T I K S I S Q Q S G A R I E L Q R N P P P N A D P N M K L F T I R G T P Q Q I D Y A R Q L I E E K I G G P V N P L G P P V P H G P H G V P G P H G P P G P P G P G T P M G P Y N P A P Y N P G P P G P A P H G P P A P Y A P Q G W G N A Y P H W Q Q Q A P P D P A K A G T D P N S A A W A A Y Y A H Y Y Q Q Q A Q P P P A A P A G A P T T T Q T N G Q G D Q Q N P A P A G Q V D Y T K A W E E Y Y K K M G Q A V P A P T G A P P G G Q P D Y S A A W A E Y Y R Q Q A A Y Y A Q T S P Q G M P Q H P P A P Q C R F D P A S I E L A L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Alternative names
Far upstream element-binding protein 1, FBP, FUSE-binding protein 1, DNA helicase V, hDH V, FUBP1
Recommended products
Recombinant Human FUBP1/FBP protein is a Human Full Length protein, in the 1 to 653 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q96AE4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A D Y S T V P P P S S G S A G G G G G G G G G G G V N D A F K D A L Q R A R Q I A A K I G G D A G T S L N S N D Y G Y G G Q K R P L E D G D Q P D A K K V A P Q N D S F G T Q L P P M H Q Q Q R S V M T E E Y K V P D G M V G F I I G R G G E Q I S R I Q Q E S G C K I Q I A P D S G G L P E R S C M L T G T P E S V Q S A K R L L D Q I V E K G R P A P G F H H G D G P G N A V Q E I M I P A S K A G L V I G K G G E T I K Q L Q E R A G V K M V M I Q D G P Q N T G A D K P L R I T G D P Y K V Q Q A K E M V L E L I R D Q G G F R E V R N E Y G S R I G G N E G I D V P I P R F A V G I V I G R N G E M I K K I Q N D A G V R I Q F K P D D G T T P E R I A Q I T G P P D R C Q H A A E I I T D L L R S V Q A G N P G G P G P G G R G R G R G Q G N W N M G P P G G L Q E F N F I V P T G K T G L I I G K G G E T I K S I S Q Q S G A R I E L Q R N P P P N A D P N M K L F T I R G T P Q Q I D Y A R Q L I E E K I G G P V N P L G P P V P H G P H G V P G P H G P P G P P G P G T P M G P Y N P A P Y N P G P P G P A P H G P P A P Y A P Q G W G N A Y P H W Q Q Q A P P D P A K A G T D P N S A A W A A Y Y A H Y Y Q Q Q A Q P P P A A P A G A P T T T Q T N G Q G D Q Q N P A P A G Q V D Y T K A W E E Y Y K K M G Q A V P A P T G A P P G G Q P D Y S A A W A E Y Y R Q Q A A Y Y A Q T S P Q G M P Q H P P A P Q C R F D P A S I E L A L
- Accession
- Q96AE4
- Protein length
- Full Length
- Amino acids
- 1 to 653
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
- Post-translational modifications
Ubiquitinated. This targets the protein for proteasome-mediated degradation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as FUBP1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The far upstream element-binding protein 1 (FUBP1) also known as FBP or FBP west is a single-stranded DNA-binding protein with a molecular mass of approximately 65 kDa. It is expressed in a range of tissues including the liver brain and hematopoietic cells. Mechanically FUBP1 regulates the transcription of genes by binding to the far upstream element (FUSE) located in proximity to transcription start sites. It plays an important role in modulating transcription factors and aids in the stability of mRNA.
- Biological function summary
FUBP1 influences cellular proliferation and differentiation. It does not form part of a larger complex but interacts with different molecules to exert its function. The protein acts as an activator of transcription fostering interaction with RNA polymerase II and other factors to elevate gene expression. FUBP1's activity helps in controlling the cell cycle aiding in the switch between proliferation and cell growth arrest as needed.
- Pathways
FUBP1 participates significantly in the c-Myc regulatory and p53 signaling pathways. In the context of the c-Myc pathway FUBP1 boosts the expression of c-Myc by stabilizing its mRNA a process also influenced by the U2 auxiliary factor (U2AF65). In the p53 signaling pathway FUBP1 indirectly influences p53's role in apoptosis and cellular stress responses as its regulatory balance shifts critical cellular processes depending on external and internal cues.
- Associated diseases and disorders
FUBP1's dysregulation relates to cancer and neurological disorders. Overexpression or aberrant regulation of FUBP1 links to tumorigenesis in cancers like liver cancer partially due to its interaction with c-Myc. Additionally mutations or alterations in FUBP1 are associated with oligodendrogliomas a type of brain tumor. Proteins such as p53 also link to these disorders due to FUBP1's involvement in complex signaling networks.
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1 product image
SDS-PAGE - Recombinant Human FUBP1/FBP protein (ab152933)
ab152933 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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Product protocols
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