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Recombinant Human FUNDC1 protein is a Human Full Length protein, in the 1 to 155 aa range, expressed in Wheat germ and suitable for ELISA, WB.

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Images

SDS-PAGE - Recombinant Human FUNDC1 protein (AB165435), expandable thumbnail

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

Biologically active

No

Amino acid sequence

M A T R N P P P Q D Y E S D D D S Y E V L D L T E Y A R R H Q W W N R V F G H S S G P M V E K Y S V A T Q I V M G G V T G W C A G F L F Q K V G K L A A T A V G G G F L L L Q I A S H S G Y V Q I D W K R V E K D V N K A K R Q I K K R A N K A A P E I N N L I E E A T E F I K Q N I V I S S G F V G G F L L G L A S

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Integral mitochondrial outer-membrane protein that mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs) (PubMed:33972548). In turn, mediates angiogenesis and neoangiogenesis through interference with intracellular Ca(2+) communication and regulation of the vascular endothelial growth factor receptor KDR/VEGFR2 expression at both mRNA and protein levels (PubMed:33972548). Acts also as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality and homeostasis, by interacting with and recruiting LC3 protein family to mitochondria (PubMed:22267086, PubMed:24671035, PubMed:24746696, PubMed:27653272). Mechanistically, recruits DRP1 at ER-mitochondria contact sites leading to DRP1 oligomerization and GTPase activity to facilitate mitochondrial fission during hypoxia (PubMed:27145933, PubMed:33978709). Additionally, plays a role in hepatic ferroptosis by interacting directly with glutathione peroxidase/GPX4 to facilitate its recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy (PubMed:36828120).

Alternative names

Recommended products

Recombinant Human FUNDC1 protein is a Human Full Length protein, in the 1 to 155 aa range, expressed in Wheat germ and suitable for ELISA, WB.

Key facts

Expression system

Wheat germ

Applications

ELISA, WB

Accession
Q8IVP5-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M A T R N P P P Q D Y E S D D D S Y E V L D L T E Y A R R H Q W W N R V F G H S S G P M V E K Y S V A T Q I V M G G V T G W C A G F L F Q K V G K L A A T A V G G G F L L L Q I A S H S G Y V Q I D W K R V E K D V N K A K R Q I K K R A N K A A P E I N N L I E E A T E F I K Q N I V I S S G F V G G F L L G L A S

Accession

Q8IVP5

Protein length

Full Length

Amino acids

1 to 155

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

General info

Function

Integral mitochondrial outer-membrane protein that mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs) (PubMed:33972548). In turn, mediates angiogenesis and neoangiogenesis through interference with intracellular Ca(2+) communication and regulation of the vascular endothelial growth factor receptor KDR/VEGFR2 expression at both mRNA and protein levels (PubMed:33972548). Acts also as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality and homeostasis, by interacting with and recruiting LC3 protein family to mitochondria (PubMed:22267086, PubMed:24671035, PubMed:24746696, PubMed:27653272). Mechanistically, recruits DRP1 at ER-mitochondria contact sites leading to DRP1 oligomerization and GTPase activity to facilitate mitochondrial fission during hypoxia (PubMed:27145933, PubMed:33978709). Additionally, plays a role in hepatic ferroptosis by interacting directly with glutathione peroxidase/GPX4 to facilitate its recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy (PubMed:36828120).

Sequence similarities

Belongs to the FUN14 family.

Post-translational modifications

Phosphorylation at Ser-13 by CK2 and at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy. Dephosphorylation is mediated by PGAM5 (PubMed:24746696). Phosphorylated by ULK1 at Ser-17 which enhances FUNDC1 binding to LC3 (PubMed:24671035).

Subcellular localisation

Mitochondrion outer membrane

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

U+00A0;

U+00A0;

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FUNDC1 also known as FUN14 domain-containing protein 1 is a protein involved in mitochondrial dynamics. It weighs approximately 15 kDa. FUNDC1 is primarily expressed in the mitochondrial outer membrane and shows high expression levels in tissues with high energy demands such as the heart and skeletal muscles. Its function is linked to the regulation of mitophagy a process that removes damaged mitochondria.

Biological function summary

FUNDC1 plays a role in the selective elimination of mitochondria through mitophagy. It acts as a receptor for damaged mitochondria binding to LC3 and enabling the autophagic machinery. FUNDC1 is not known to be part of a larger protein complex but it interacts with other mitophagy-related proteins such as PGAM5 and ULK1 to facilitate mitochondrial clearance.

Pathways

FUNDC1 participates in the mitophagy pathway and the broader mitochondrial quality control pathway. In the mitophagy pathway its interaction with proteins like LC3 facilitates the engulfment of dysfunctional mitochondria by autophagosomes. The relationship of FUNDC1 with ULK1 highlights its role in the initiation of the mitophagy signaling cascade ensuring mitochondrial integrity and cellular homeostasis.

Associated diseases and disorders

FUNDC1 has associations with neurodegenerative diseases and cardiovascular disorders. Defects in the mitophagy process where FUNDC1 plays a significant role can contribute to the development of Parkinson's disease. Furthermore its connection to cardiovascular diseases involves interactions with BCL2L1 a protein that regulates apoptosis in heart tissue under stress conditions. Dysfunctional FUNDC1 activity may lead to the accumulation of damaged mitochondria contributing to cellular dysfunction observed in these diseases.

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1 product image

  • SDS-PAGE - Recombinant Human FUNDC1 protein (ab165435), expandable thumbnail

    SDS-PAGE - Recombinant Human FUNDC1 protein (ab165435)

    ab165435 on a 12.5% SDS-PAGE stained with Coomassie Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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