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AB114702

Recombinant Human FUT8 protein (GST tag N-Terminus)

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Recombinant Human FUT8 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 575 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Alpha1-6FucT, Fucosyltransferase 8, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8

1 Images
SDS-PAGE - Recombinant Human FUT8 protein (GST tag N-Terminus) (AB114702)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FUT8 protein (GST tag N-Terminus) (AB114702)

12.5% SDS-PAGE showing ab114702 at approximately 89.32 kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q9BYC5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB198741

Anti-FUT8 antibody

Western blot - Anti-FUT8 antibody (AB198741)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB174314

Anti-PAPP A antibody [EPR12897]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (AB174314)

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  • 0
Assay Kits

AB313609

Human FUT8 ELISA Kit

Sandwich ELISA - Human FUT8 ELISA Kit (AB313609)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Sequence info

[{"sequence":"MRPWTGSWRWIMLILFAWGTLLFYIGGHLVRDNDHPDHSSRELSKILAKLERLKQQNEDLRRMAESLRIPEGPIDQGPAIGRVRVLEEQLVKAKEQIENYKKQTRNGLGKDHEILRRRIENGAKELWFFLQSELKKLKNLEGNELQRHADEFLLDLGHHERSIMTDLYYLSQTDGAGDWREKEAKDLTELVQRRITYLQNPKDCSKAKKLVCNINKGCGYGCQLHHVVYCFMIAYGTQRTLILESQNWRYATGGWETVFRPVSETCTDRSGISTGHWSGEVKDKNVQVVELPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK","proteinLength":"Full Length","predictedMolecularWeight":"89.32 kDa","actualMolecularWeight":null,"aminoAcidEnd":575,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9BYC5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FUT8 also known as alpha-(16)-fucosyltransferase represents an important enzyme responsible for transferring fucose from GDP-fucose to the innermost N-acetylglucosamine of N-glycans producing core fucosylation. The molecular mass of FUT8 is approximately 60 kDa. It expresses ubiquitously across various tissues with elevated levels seen in the liver lung and placenta. Researchers frequently study FUT8 due to its significance in glycosylation processes.
Biological function summary

FUT8 impacts numerous cellular activities by modulating glycan branching on glycoproteins. It functions independently and is not directly part of a larger protein complex. FUT8's activity influences cell-cell interactions signaling pathways and receptor functions. It plays an important part in maintaining cellular homeostasis and influences immune responses and cell growth as affected by glycosylation patterns on the cell surface and extracellular matrix.

Pathways

FUT8 is a critical component of the N-glycan biosynthesis pathway where it regulates the addition of fucose to glycans. This process affects protein folding and stability. The enzyme interacts with important glycoproteins like EGFR and integrins impacting signaling pathways such as the Wnt and TGF-beta pathways. Altered FUT8 activity can modify pathways and relationships with other proteins changing cellular responses.

FUT8 has connections to cancers and liver fibrosis due to its role in modifying glycans and subsequently influencing cellular communication and adhesion. Abnormal core fucosylation affects proteins like alpha-fetoprotein in liver cancer which might serve as a biomarker. Furthermore changes in FUT8 activity and expression could relate to epidermal growth factor receptor (EGFR) signaling disruptions promoting tumor proliferation. Scientists consider FUT8 a potential therapeutic target in these diseases.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans (PubMed : 17172260, PubMed : 29304374, PubMed : 36280670, PubMed : 9133635). Fucosylates the reducing GlcNAc residue in complex-type N-glycans attached on the fragment crystallizable (Fc) of IgGs. Fully converts Fc glycoforms containing one or two terminal GlcNAc moieties (G0-GlcNAc and G0) (PubMed : 36280670).

Sequence similarities

Belongs to the glycosyltransferase 23 family.

Post-translational modifications

Tyrosine phosphorylated by PKDCC/VLK.

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Product protocols

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Target data

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans (PubMed : 17172260, PubMed : 29304374, PubMed : 36280670, PubMed : 9133635). Fucosylates the reducing GlcNAc residue in complex-type N-glycans attached on the fragment crystallizable (Fc) of IgGs. Fully converts Fc glycoforms containing one or two terminal GlcNAc moieties (G0-GlcNAc and G0) (PubMed : 36280670).
See full target information FUT8
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Product promise

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