Recombinant Human FXI Protein Standard
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- Domain: Apple 1, Apple 2, Apple 3, Apple 4, Peptidase S1
- Localization: Extracellular
- Proximate region: Full length
- Tag free
- >80% SDS-PAGE
- SDS-PAGE
Lab
SDS-PAGE - Recombinant Human FXI Protein Standard (AB327829)
SDS-PAGE analysis of ab327829 under reducing conditions for 2ug protein.
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Form
Purification technique
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
General info
Function
Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Post-translational modifications
N-glycosylated on both chains. N-glycosylated sites mainly consist of nonfucosylated sialylated biantennary (in high abundance) and/or triantennary (in low abundance) complex structures. Glycosylation at Asn-163 uses a rare non-canonical Asn-X-Cys glycosite.. Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen. Activated by F12 (activated); the presence of negatively charged surfaces accelerates activation (PubMed:2019570, PubMed:8427954). Activated by F2 (thrombin); the presence of negatively charged surfaces, such as polyphosphate and dextran sulfate, strongly accelerates activation (PubMed:2019570, PubMed:21976677). Autoactivated; the presence of negatively charged surfaces, such as polyphosphate and dextran sulfate, accelerates autoactivation and autolysis (PubMed:2019570, PubMed:21976677).
Target data
Alternative Names
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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